Urea and lactate determined in 1-μL whole-blood samples with a miniaturized thermal biosensor
(1994) In Clinical Chemistry 40(12). p.2282-2287- Abstract
A miniaturized flow-injected thermal biosensor was developed for the determination of urea and L-lactate in undiluted blood in 1-μL samples. The sensor employed a small enzyme column constructed of stainless steel tubing and microbead thermistors. Urease and lactate oxidase/catalase were separately immobilized onto controlled-pore glass beads, which, in turn, were charged into the enzyme column. With a flow rate of 70 μL/min, linear analytical ranges from 0.2 to at least 50 mmol/L and 0.2 to 14 mmol/L were obtained for urea and lactate, respectively. The relative standard deviations (CVs) for measurements of analyte in buffer were 0.91% for urea and 1.84% for lactate. For urea in whole blood, the CV for 50 determinations was 4.1%.... (More)
A miniaturized flow-injected thermal biosensor was developed for the determination of urea and L-lactate in undiluted blood in 1-μL samples. The sensor employed a small enzyme column constructed of stainless steel tubing and microbead thermistors. Urease and lactate oxidase/catalase were separately immobilized onto controlled-pore glass beads, which, in turn, were charged into the enzyme column. With a flow rate of 70 μL/min, linear analytical ranges from 0.2 to at least 50 mmol/L and 0.2 to 14 mmol/L were obtained for urea and lactate, respectively. The relative standard deviations (CVs) for measurements of analyte in buffer were 0.91% for urea and 1.84% for lactate. For urea in whole blood, the CV for 50 determinations was 4.1%. Contrived samples containing various concentrations of urea and L-lactate in whole blood were determined with this sensor and with a spectrophotometric method. Comparisons of the results gave correlation coefficients of 0.989 and 0.984 for 30 blood urea and 30 blood lactate assays in concentrations ranging from 4 to 20.9 mmol/L and from 1.7 to 12.7 mmol/L, respectively.
(Less)
- author
- Xie, Bin LU ; Harborn, Ulrika ; Mecklenburg, Michael and Danielsson, Bengt LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical Chemistry
- volume
- 40
- issue
- 12
- pages
- 6 pages
- publisher
- American Association for Clinical Chemistry
- external identifiers
-
- scopus:0028670525
- pmid:7988016
- ISSN
- 0009-9147
- DOI
- 10.1093/clinchem/40.12.2282
- language
- English
- LU publication?
- yes
- id
- 6543f523-80a9-476e-bab4-7414c07df4a7
- date added to LUP
- 2021-10-27 15:55:37
- date last changed
- 2024-05-19 17:23:05
@article{6543f523-80a9-476e-bab4-7414c07df4a7,
abstract = {{<p>A miniaturized flow-injected thermal biosensor was developed for the determination of urea and L-lactate in undiluted blood in 1-μL samples. The sensor employed a small enzyme column constructed of stainless steel tubing and microbead thermistors. Urease and lactate oxidase/catalase were separately immobilized onto controlled-pore glass beads, which, in turn, were charged into the enzyme column. With a flow rate of 70 μL/min, linear analytical ranges from 0.2 to at least 50 mmol/L and 0.2 to 14 mmol/L were obtained for urea and lactate, respectively. The relative standard deviations (CVs) for measurements of analyte in buffer were 0.91% for urea and 1.84% for lactate. For urea in whole blood, the CV for 50 determinations was 4.1%. Contrived samples containing various concentrations of urea and L-lactate in whole blood were determined with this sensor and with a spectrophotometric method. Comparisons of the results gave correlation coefficients of 0.989 and 0.984 for 30 blood urea and 30 blood lactate assays in concentrations ranging from 4 to 20.9 mmol/L and from 1.7 to 12.7 mmol/L, respectively.</p>}},
author = {{Xie, Bin and Harborn, Ulrika and Mecklenburg, Michael and Danielsson, Bengt}},
issn = {{0009-9147}},
language = {{eng}},
number = {{12}},
pages = {{2282--2287}},
publisher = {{American Association for Clinical Chemistry}},
series = {{Clinical Chemistry}},
title = {{Urea and lactate determined in 1-μL whole-blood samples with a miniaturized thermal biosensor}},
url = {{http://dx.doi.org/10.1093/clinchem/40.12.2282}},
doi = {{10.1093/clinchem/40.12.2282}},
volume = {{40}},
year = {{1994}},
}