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Structural organisation of prolamellar bodies (PLB) isolated from Zea mays. Parallel TEM, SAXS and absorption spectra measurements on samples subjected to freeze-thaw, reduced pH and high-salt perturbation

Selstam, Eva; Schelin, Jenny LU ; Williams, W. Patrick and Brain, Anthony P. R. (2007) In Biochimica et Biophysica Acta - Biomembranes 1768(9). p.2235-2245
Abstract
Well-organised PLB gives rise to a X-ray diffraction pattern overlaid by a scattering pattern arising from individual tubules within less wellorganised regions of the lattice. TEM and SAXS measurements were used to characterise the structural changes in PLB subjected to perturbation by freeze-thaw, exposure to pH 6.5, or resuspension in high-salt media. Comparison of SAXS patterns measured, before and after structural perturbation allows the separation of the contributions from ordered and disordered PLB. The diffraction pattern is shown to be based on a diamond cubic (Fd3m) lattice of unit cell a= 78 nm. Freeze-thaw and high-salt disruption lead to the breakdown of ordered PLB into disordered tubules of similar dimensions to those making... (More)
Well-organised PLB gives rise to a X-ray diffraction pattern overlaid by a scattering pattern arising from individual tubules within less wellorganised regions of the lattice. TEM and SAXS measurements were used to characterise the structural changes in PLB subjected to perturbation by freeze-thaw, exposure to pH 6.5, or resuspension in high-salt media. Comparison of SAXS patterns measured, before and after structural perturbation allows the separation of the contributions from ordered and disordered PLB. The diffraction pattern is shown to be based on a diamond cubic (Fd3m) lattice of unit cell a= 78 nm. Freeze-thaw and high-salt disruption lead to the breakdown of ordered PLB into disordered tubules of similar dimensions to those making up the original PLB lattice. Their scattering patterns suggest that they are approximately 26 nm in diameter with a central lumen about 16 nm in diameter. The tubules formed at pH 6.5 are appreciably narrower, probably reflecting changes in the pattern of ionisation of charged groups at the membrane surface. Absorption spectra of PLB in media containing different concentrations of salts indicated that the structural and spectral changes are related. NADPH, have a significant role in the protection of POR-PChfide(650) but to have only a relatively small effect on the preservation of PLB organisation indicating that the retention of POR-PChlide(650) in isolated PLB preparations is a poor guide to their structural integrity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
X-ray, scattering, electron microscopy, chlorophyllide, protochlorophyllide, protochlorophyllide oxidoreductase, prolamellar body
in
Biochimica et Biophysica Acta - Biomembranes
volume
1768
issue
9
pages
2235 - 2245
publisher
Elsevier
external identifiers
  • wos:000250040700022
  • scopus:34548484106
ISSN
0005-2736
DOI
10.1016/j.bbamem.2007.05.005
language
English
LU publication?
yes
id
d5d49aeb-ae33-4c55-92a1-a6b43e31553d (old id 655448)
date added to LUP
2007-12-18 14:44:35
date last changed
2017-10-01 04:53:39
@article{d5d49aeb-ae33-4c55-92a1-a6b43e31553d,
  abstract     = {Well-organised PLB gives rise to a X-ray diffraction pattern overlaid by a scattering pattern arising from individual tubules within less wellorganised regions of the lattice. TEM and SAXS measurements were used to characterise the structural changes in PLB subjected to perturbation by freeze-thaw, exposure to pH 6.5, or resuspension in high-salt media. Comparison of SAXS patterns measured, before and after structural perturbation allows the separation of the contributions from ordered and disordered PLB. The diffraction pattern is shown to be based on a diamond cubic (Fd3m) lattice of unit cell a= 78 nm. Freeze-thaw and high-salt disruption lead to the breakdown of ordered PLB into disordered tubules of similar dimensions to those making up the original PLB lattice. Their scattering patterns suggest that they are approximately 26 nm in diameter with a central lumen about 16 nm in diameter. The tubules formed at pH 6.5 are appreciably narrower, probably reflecting changes in the pattern of ionisation of charged groups at the membrane surface. Absorption spectra of PLB in media containing different concentrations of salts indicated that the structural and spectral changes are related. NADPH, have a significant role in the protection of POR-PChfide(650) but to have only a relatively small effect on the preservation of PLB organisation indicating that the retention of POR-PChlide(650) in isolated PLB preparations is a poor guide to their structural integrity.},
  author       = {Selstam, Eva and Schelin, Jenny and Williams, W. Patrick and Brain, Anthony P. R.},
  issn         = {0005-2736},
  keyword      = {X-ray,scattering,electron microscopy,chlorophyllide,protochlorophyllide,protochlorophyllide oxidoreductase,prolamellar body},
  language     = {eng},
  number       = {9},
  pages        = {2235--2245},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Biomembranes},
  title        = {Structural organisation of prolamellar bodies (PLB) isolated from Zea mays. Parallel TEM, SAXS and absorption spectra measurements on samples subjected to freeze-thaw, reduced pH and high-salt perturbation},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2007.05.005},
  volume       = {1768},
  year         = {2007},
}