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In vitro evolution of a fungal laccase in high concentrations of organic cosolvents

Zumarraga, Miren; Bulter, Thomas; Shleev, Sergey LU ; Polaina, Julio; Martinez-Arias, Arturo; Plow, Francisco J.; Ballesteros, Antonio and Alcalde, Miguel (2007) In Chemistry and Biology 14(9). p.1052-1064
Abstract
Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry... (More)
Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry of catalytic coppers varied slightly during the course of the molecular evolution. Some mutations at the protein surface stabilized the laccase by allowing additional electrostatic and hydrogen bonding to occur. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CHEMBIOL
in
Chemistry and Biology
volume
14
issue
9
pages
1052 - 1064
publisher
Cell Press
external identifiers
  • wos:000249913600012
  • scopus:34548605576
ISSN
1879-1301
DOI
10.1016/j.chembiol.2007.08.010
language
English
LU publication?
yes
id
6fcdc703-6d3b-4805-90dd-010f3258dabe (old id 655771)
date added to LUP
2007-12-19 11:11:32
date last changed
2017-07-30 03:37:50
@article{6fcdc703-6d3b-4805-90dd-010f3258dabe,
  abstract     = {Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry of catalytic coppers varied slightly during the course of the molecular evolution. Some mutations at the protein surface stabilized the laccase by allowing additional electrostatic and hydrogen bonding to occur.},
  author       = {Zumarraga, Miren and Bulter, Thomas and Shleev, Sergey and Polaina, Julio and Martinez-Arias, Arturo and Plow, Francisco J. and Ballesteros, Antonio and Alcalde, Miguel},
  issn         = {1879-1301},
  keyword      = {CHEMBIOL},
  language     = {eng},
  number       = {9},
  pages        = {1052--1064},
  publisher    = {Cell Press},
  series       = {Chemistry and Biology},
  title        = {In vitro evolution of a fungal laccase in high concentrations of organic cosolvents},
  url          = {http://dx.doi.org/10.1016/j.chembiol.2007.08.010},
  volume       = {14},
  year         = {2007},
}