In vitro evolution of a fungal laccase in high concentrations of organic cosolvents
(2007) In Chemistry and Biology 14(9). p.1052-1064- Abstract
- Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry... (More)
- Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry of catalytic coppers varied slightly during the course of the molecular evolution. Some mutations at the protein surface stabilized the laccase by allowing additional electrostatic and hydrogen bonding to occur. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/655771
- author
- Zumarraga, Miren ; Bulter, Thomas ; Shleev, Sergey LU ; Polaina, Julio ; Martinez-Arias, Arturo ; Plow, Francisco J. ; Ballesteros, Antonio and Alcalde, Miguel
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CHEMBIOL
- in
- Chemistry and Biology
- volume
- 14
- issue
- 9
- pages
- 1052 - 1064
- publisher
- Cell Press
- external identifiers
-
- wos:000249913600012
- scopus:34548605576
- ISSN
- 1879-1301
- DOI
- 10.1016/j.chembiol.2007.08.010
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 6fcdc703-6d3b-4805-90dd-010f3258dabe (old id 655771)
- date added to LUP
- 2016-04-01 11:53:21
- date last changed
- 2022-03-05 07:57:39
@article{6fcdc703-6d3b-4805-90dd-010f3258dabe, abstract = {{Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry of catalytic coppers varied slightly during the course of the molecular evolution. Some mutations at the protein surface stabilized the laccase by allowing additional electrostatic and hydrogen bonding to occur.}}, author = {{Zumarraga, Miren and Bulter, Thomas and Shleev, Sergey and Polaina, Julio and Martinez-Arias, Arturo and Plow, Francisco J. and Ballesteros, Antonio and Alcalde, Miguel}}, issn = {{1879-1301}}, keywords = {{CHEMBIOL}}, language = {{eng}}, number = {{9}}, pages = {{1052--1064}}, publisher = {{Cell Press}}, series = {{Chemistry and Biology}}, title = {{In vitro evolution of a fungal laccase in high concentrations of organic cosolvents}}, url = {{http://dx.doi.org/10.1016/j.chembiol.2007.08.010}}, doi = {{10.1016/j.chembiol.2007.08.010}}, volume = {{14}}, year = {{2007}}, }