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Hypothermia Prevents the Ischemia‐Induced Translocation and Inhibition of Protein Kinase C in the Rat Striatum

Cardell, Monika LU ; Boris‐Möller, Fredrik and Wieloch, Tadeusz LU (1991) In Journal of Neurochemistry 57(5). p.1814-1817
Abstract

: The effect of hypothermia on the ischemia‐induced changes in the subcellular distribution of protein kinase C (PKC)(γ), ‐(βII), and ‐(α) and the activity of PKC was studied in striatal homogenates of rats subjected to 20 min of cerebral ischemia. The effect of post‐ischemic cooling was also studied. During normothermic ischemia, PKC(γ) and ‐(βII) increased 3.9‐and 2.9‐fold, respectively, in the particulate fraction, signifying a translocation of PKC to cell membranes. The levels of PKC(α) did not change significantly. PKC activity decreased during ischemia by 52% and 47% (p < 0.05) in the paniculate and cytosolic fractions, respectively, and remained inhibited for the 1 h recovery period. In hypothermic... (More)

: The effect of hypothermia on the ischemia‐induced changes in the subcellular distribution of protein kinase C (PKC)(γ), ‐(βII), and ‐(α) and the activity of PKC was studied in striatal homogenates of rats subjected to 20 min of cerebral ischemia. The effect of post‐ischemic cooling was also studied. During normothermic ischemia, PKC(γ) and ‐(βII) increased 3.9‐and 2.9‐fold, respectively, in the particulate fraction, signifying a translocation of PKC to cell membranes. The levels of PKC(α) did not change significantly. PKC activity decreased during ischemia by 52% and 47% (p < 0.05) in the paniculate and cytosolic fractions, respectively, and remained inhibited for the 1 h recovery period. In hypothermic animals, there was no evidence of translocation, and the inhibition of PKC activity was completely abolished. Hypothermia induced in the recovery phase, however, did not affect PKC distribution or activity. The protective effect of intraischemic hypothermia may in part be due to the prevention of the ischemia‐induced translocation and subsequent downregulation of PKC, possibly through a temperature‐dependent modification of the cell membranes.

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author
; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Activity, Hypothermia, Ischemia, Protein kinase C, Subcellular distribution
in
Journal of Neurochemistry
volume
57
issue
5
pages
1814 - 1817
publisher
Wiley-Blackwell
external identifiers
  • pmid:1919586
  • scopus:0025936317
ISSN
0022-3042
DOI
10.1111/j.1471-4159.1991.tb06387.x
language
English
LU publication?
no
id
65645a3d-ef85-40d3-81b3-e84d6ccac30a
date added to LUP
2019-06-13 16:25:30
date last changed
2024-01-01 10:19:42
@article{65645a3d-ef85-40d3-81b3-e84d6ccac30a,
  abstract     = {{<p>: The effect of hypothermia on the ischemia‐induced changes in the subcellular distribution of protein kinase C (PKC)(γ), ‐(β<sub>II</sub>), and ‐(α) and the activity of PKC was studied in striatal homogenates of rats subjected to 20 min of cerebral ischemia. The effect of post‐ischemic cooling was also studied. During normothermic ischemia, PKC(γ) and ‐(β<sub>II</sub>) increased 3.9‐and 2.9‐fold, respectively, in the particulate fraction, signifying a translocation of PKC to cell membranes. The levels of PKC(α) did not change significantly. PKC activity decreased during ischemia by 52% and 47% (p &lt; 0.05) in the paniculate and cytosolic fractions, respectively, and remained inhibited for the 1 h recovery period. In hypothermic animals, there was no evidence of translocation, and the inhibition of PKC activity was completely abolished. Hypothermia induced in the recovery phase, however, did not affect PKC distribution or activity. The protective effect of intraischemic hypothermia may in part be due to the prevention of the ischemia‐induced translocation and subsequent downregulation of PKC, possibly through a temperature‐dependent modification of the cell membranes.</p>}},
  author       = {{Cardell, Monika and Boris‐Möller, Fredrik and Wieloch, Tadeusz}},
  issn         = {{0022-3042}},
  keywords     = {{Activity; Hypothermia; Ischemia; Protein kinase C; Subcellular distribution}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1814--1817}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of Neurochemistry}},
  title        = {{Hypothermia Prevents the Ischemia‐Induced Translocation and Inhibition of Protein Kinase C in the Rat Striatum}},
  url          = {{http://dx.doi.org/10.1111/j.1471-4159.1991.tb06387.x}},
  doi          = {{10.1111/j.1471-4159.1991.tb06387.x}},
  volume       = {{57}},
  year         = {{1991}},
}