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Evaluation of quantitative thin layer chromatography using staining reagents

Johnsson, Richard LU ; Traff, Gustav; Sundén, Martin LU and Ellervik, Ulf LU (2007) In Journal of Chromatography A 1164(1-2). p.298-305
Abstract
Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
quantification, TLC, image analysis software, staining reagents
in
Journal of Chromatography A
volume
1164
issue
1-2
pages
298 - 305
publisher
Elsevier
external identifiers
  • wos:000249471900037
  • scopus:34548037947
ISSN
0021-9673
DOI
10.1016/j.chroma.2007.07.029
language
English
LU publication?
yes
id
771476c7-1dcb-42de-bac8-b2a7cfe708ef (old id 656570)
date added to LUP
2007-12-13 13:54:29
date last changed
2017-06-11 04:23:47
@article{771476c7-1dcb-42de-bac8-b2a7cfe708ef,
  abstract     = {Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Johnsson, Richard and Traff, Gustav and Sundén, Martin and Ellervik, Ulf},
  issn         = {0021-9673},
  keyword      = {quantification,TLC,image analysis software,staining reagents},
  language     = {eng},
  number       = {1-2},
  pages        = {298--305},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Evaluation of quantitative thin layer chromatography using staining reagents},
  url          = {http://dx.doi.org/10.1016/j.chroma.2007.07.029},
  volume       = {1164},
  year         = {2007},
}