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Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction

Torbet, Jim; Malbouyres, Marilyne; Builles, Nicolas; Justin, Virginie; Roulet, Muriel; Damour, Odile; Oldberg, Åke LU ; Ruggieo, Florence and Hulmes, David J. S. (2007) In Biomaterials 28(29). p.4268-4276
Abstract
The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibrils. This study demonstrates that such a scaffold can be built up using magnetic alignment. By allowing neutralized acid-soluble type I collagen to gel in a horizontal magnetic field (7 T) and by combining a series of gelation-rotation-gelation cycles, a scaffold of orthogonal lamellac composed of aligned collagen fibrils has been formed. Although initially dilute, the gels can be concentrated without noticeable loss in... (More)
The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibrils. This study demonstrates that such a scaffold can be built up using magnetic alignment. By allowing neutralized acid-soluble type I collagen to gel in a horizontal magnetic field (7 T) and by combining a series of gelation-rotation-gelation cycles, a scaffold of orthogonal lamellac composed of aligned collagen fibrils has been formed. Although initially dilute, the gels can be concentrated without noticeable loss in orientation. The gels are translucent but their transparency can be greatly improved by the addition of proteoglycans to the gel-forming solution. Keratocytes align by contact guidance along the direction of collagen fibrils and respect the orthogonal design of the collagen template as they penetrate into the bulk of the 3D matrix. The scaffold is a significant step towards the creation of a corneal substitute with properties resembling those of native corneal stroma. (c) 2007 Elsevier Ltd. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
stroma-like scaffold, magnetic alignment, keratocytes, proteoglycans, corneal stroma reconstruction, collagen
in
Biomaterials
volume
28
issue
29
pages
4268 - 4276
publisher
Elsevier
external identifiers
  • wos:000249605400011
  • scopus:34547697458
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2007.05.024
language
English
LU publication?
yes
id
cfb8f57a-dce7-4409-8e3c-cf54830979f0 (old id 656717)
date added to LUP
2008-01-03 10:48:10
date last changed
2017-10-08 03:30:49
@article{cfb8f57a-dce7-4409-8e3c-cf54830979f0,
  abstract     = {The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibrils. This study demonstrates that such a scaffold can be built up using magnetic alignment. By allowing neutralized acid-soluble type I collagen to gel in a horizontal magnetic field (7 T) and by combining a series of gelation-rotation-gelation cycles, a scaffold of orthogonal lamellac composed of aligned collagen fibrils has been formed. Although initially dilute, the gels can be concentrated without noticeable loss in orientation. The gels are translucent but their transparency can be greatly improved by the addition of proteoglycans to the gel-forming solution. Keratocytes align by contact guidance along the direction of collagen fibrils and respect the orthogonal design of the collagen template as they penetrate into the bulk of the 3D matrix. The scaffold is a significant step towards the creation of a corneal substitute with properties resembling those of native corneal stroma. (c) 2007 Elsevier Ltd. All rights reserved.},
  author       = {Torbet, Jim and Malbouyres, Marilyne and Builles, Nicolas and Justin, Virginie and Roulet, Muriel and Damour, Odile and Oldberg, Åke and Ruggieo, Florence and Hulmes, David J. S.},
  issn         = {1878-5905},
  keyword      = {stroma-like scaffold,magnetic alignment,keratocytes,proteoglycans,corneal stroma reconstruction,collagen},
  language     = {eng},
  number       = {29},
  pages        = {4268--4276},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2007.05.024},
  volume       = {28},
  year         = {2007},
}