Advanced

Bi-enzyme biosensor based on NAD(+)- and glutathione-dependent recombinant formaldehyde dehydrogenase and diaphorase for formaldehyde assay

Nikitina, Oxana; Shleev, Sergey LU ; Gayda, Galina; Demkiv, Olha; Gonchar, Mykhaylo; Gorton, Lo LU ; Csöregi, Elisabeth LU and Nistor, Mihaela LU (2007) In Sensors and Actuators B: Chemical 125(1). p.1-9
Abstract
The present work reports on the development of a bi-enzyme biosensor using diaphorase from Bacillus stearothermophilus, nicotinamide adenine dinucleotide (NAD(+))- and glutathione (GSH)-dependent formaldehyde dehydrogenase (FDH) from the genetically-engineered methylotrophic yeast Hansenula polymorpha as bio-recognition elements. The sensor architecture comprises a first layer containing diaphorase cross-linked with an osmium complex-modified redox polymer (poly(vinylpyridine)-[osmium-(N,N'-methylated-2,2'-biimidalzole)(3)](3+/ 2+) complex). On its top, a second layer was formed by additional cross-linking of FDH with poly(ethylene glycol)(400)diglycidyl ether. The sensor's architecture was optimized with respect to efficient electron... (More)
The present work reports on the development of a bi-enzyme biosensor using diaphorase from Bacillus stearothermophilus, nicotinamide adenine dinucleotide (NAD(+))- and glutathione (GSH)-dependent formaldehyde dehydrogenase (FDH) from the genetically-engineered methylotrophic yeast Hansenula polymorpha as bio-recognition elements. The sensor architecture comprises a first layer containing diaphorase cross-linked with an osmium complex-modified redox polymer (poly(vinylpyridine)-[osmium-(N,N'-methylated-2,2'-biimidalzole)(3)](3+/ 2+) complex). On its top, a second layer was formed by additional cross-linking of FDH with poly(ethylene glycol)(400)diglycidyl ether. The sensor's architecture was optimized with respect to efficient electron transfer and stability of the enzyme(s). The characteristics of the optimized formaldehyde biosensor were: sensitivity 22 A m(-2) M-1, detection limit 32 mu M, and linear dynamic range 50-500 mu M. (c) 2007 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amperometry, biosensor, redox polymer, os, formaldehyde, diaphorase, recombinant formaldehyde dehydrogenase
in
Sensors and Actuators B: Chemical
volume
125
issue
1
pages
1 - 9
publisher
Elsevier
external identifiers
  • wos:000248516100001
  • scopus:34347389895
ISSN
0925-4005
DOI
10.1016/j.snb.2007.01.025
language
English
LU publication?
yes
id
1857eb8b-0788-4658-b4eb-f52c3c3267fb (old id 656880)
date added to LUP
2007-12-18 14:01:57
date last changed
2017-06-11 04:29:58
@article{1857eb8b-0788-4658-b4eb-f52c3c3267fb,
  abstract     = {The present work reports on the development of a bi-enzyme biosensor using diaphorase from Bacillus stearothermophilus, nicotinamide adenine dinucleotide (NAD(+))- and glutathione (GSH)-dependent formaldehyde dehydrogenase (FDH) from the genetically-engineered methylotrophic yeast Hansenula polymorpha as bio-recognition elements. The sensor architecture comprises a first layer containing diaphorase cross-linked with an osmium complex-modified redox polymer (poly(vinylpyridine)-[osmium-(N,N'-methylated-2,2'-biimidalzole)(3)](3+/ 2+) complex). On its top, a second layer was formed by additional cross-linking of FDH with poly(ethylene glycol)(400)diglycidyl ether. The sensor's architecture was optimized with respect to efficient electron transfer and stability of the enzyme(s). The characteristics of the optimized formaldehyde biosensor were: sensitivity 22 A m(-2) M-1, detection limit 32 mu M, and linear dynamic range 50-500 mu M. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Nikitina, Oxana and Shleev, Sergey and Gayda, Galina and Demkiv, Olha and Gonchar, Mykhaylo and Gorton, Lo and Csöregi, Elisabeth and Nistor, Mihaela},
  issn         = {0925-4005},
  keyword      = {amperometry,biosensor,redox polymer,os,formaldehyde,diaphorase,recombinant formaldehyde dehydrogenase},
  language     = {eng},
  number       = {1},
  pages        = {1--9},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Bi-enzyme biosensor based on NAD(+)- and glutathione-dependent recombinant formaldehyde dehydrogenase and diaphorase for formaldehyde assay},
  url          = {http://dx.doi.org/10.1016/j.snb.2007.01.025},
  volume       = {125},
  year         = {2007},
}