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Sulfhydryl oxidase modified composite electrode for the detection of reduced thiolic compounds

Timur, Suna; Odaci, Dilek; Dincer, Ayse; Zihnioglu, Figen; Telefoncu, Azmi and Gorton, Lo LU (2007) In Sensors and Actuators B: Chemical 125(1). p.234-239
Abstract
Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, DL-dithiotreitol) were separated by HPLC and the performance of the sulfhydril... (More)
Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, DL-dithiotreitol) were separated by HPLC and the performance of the sulfhydril oxidase SOX biosensor as a post-column electrochemical detector was investigated for the analysis of these compounds. All data were given as a comparison of two systems; biosensor and diode array detector (DAD). (c) 2007 Elsevier B.V. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
post-column enzyme, reduced thiols, biosensor, sulfhydril oxidase (SOX), based biosensor
in
Sensors and Actuators B: Chemical
volume
125
issue
1
pages
234 - 239
publisher
Elsevier
external identifiers
  • wos:000248516100035
  • scopus:34347361440
ISSN
0925-4005
DOI
10.1016/j.snb.2007.02.025
language
English
LU publication?
yes
id
1b050f43-49e5-49ee-914c-204bcd13bc7c (old id 656889)
date added to LUP
2008-01-03 10:43:42
date last changed
2017-01-01 06:59:36
@article{1b050f43-49e5-49ee-914c-204bcd13bc7c,
  abstract     = {Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, DL-dithiotreitol) were separated by HPLC and the performance of the sulfhydril oxidase SOX biosensor as a post-column electrochemical detector was investigated for the analysis of these compounds. All data were given as a comparison of two systems; biosensor and diode array detector (DAD). (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Timur, Suna and Odaci, Dilek and Dincer, Ayse and Zihnioglu, Figen and Telefoncu, Azmi and Gorton, Lo},
  issn         = {0925-4005},
  keyword      = {post-column enzyme,reduced thiols,biosensor,sulfhydril oxidase (SOX),based biosensor},
  language     = {eng},
  number       = {1},
  pages        = {234--239},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Sulfhydryl oxidase modified composite electrode for the detection of reduced thiolic compounds},
  url          = {http://dx.doi.org/10.1016/j.snb.2007.02.025},
  volume       = {125},
  year         = {2007},
}