Sulfhydryl oxidase modified composite electrode for the detection of reduced thiolic compounds
(2007) In Sensors and Actuators B: Chemical 125(1). p.234-239- Abstract
- Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, DL-dithiotreitol) were separated by HPLC and the performance of the sulfhydril... (More)
- Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, DL-dithiotreitol) were separated by HPLC and the performance of the sulfhydril oxidase SOX biosensor as a post-column electrochemical detector was investigated for the analysis of these compounds. All data were given as a comparison of two systems; biosensor and diode array detector (DAD). (c) 2007 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/656889
- author
- Timur, Suna ; Odaci, Dilek ; Dincer, Ayse ; Zihnioglu, Figen ; Telefoncu, Azmi and Gorton, Lo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- post-column enzyme, reduced thiols, biosensor, sulfhydril oxidase (SOX), based biosensor
- in
- Sensors and Actuators B: Chemical
- volume
- 125
- issue
- 1
- pages
- 234 - 239
- publisher
- Elsevier
- external identifiers
-
- wos:000248516100035
- scopus:34347361440
- ISSN
- 0925-4005
- DOI
- 10.1016/j.snb.2007.02.025
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 1b050f43-49e5-49ee-914c-204bcd13bc7c (old id 656889)
- date added to LUP
- 2016-04-01 16:14:12
- date last changed
- 2022-01-28 18:17:37
@article{1b050f43-49e5-49ee-914c-204bcd13bc7c, abstract = {{Thiol (SH) groups play a remarkable role in the cell with their redox state affecting the activity and structure of many enzymes, receptors and transcription factors. In the present work, carbon paste electrodes modified with sulfhydryl oxidase (SOX) were prepared. The proposed biosensor was based on the monitoring of the produced peroxide concentration that correlates with the concentration of reduced thiols during the enzymatic reaction. As well as analytical characteristics, optimization in terms of amount of enzyme, pH and temperature were carried out. After that, thiolic compounds (reduced/oxidized glutathione, cysteine, homocysteine, N-acetylcysteine, DL-dithiotreitol) were separated by HPLC and the performance of the sulfhydril oxidase SOX biosensor as a post-column electrochemical detector was investigated for the analysis of these compounds. All data were given as a comparison of two systems; biosensor and diode array detector (DAD). (c) 2007 Elsevier B.V. All rights reserved.}}, author = {{Timur, Suna and Odaci, Dilek and Dincer, Ayse and Zihnioglu, Figen and Telefoncu, Azmi and Gorton, Lo}}, issn = {{0925-4005}}, keywords = {{post-column enzyme; reduced thiols; biosensor; sulfhydril oxidase (SOX); based biosensor}}, language = {{eng}}, number = {{1}}, pages = {{234--239}}, publisher = {{Elsevier}}, series = {{Sensors and Actuators B: Chemical}}, title = {{Sulfhydryl oxidase modified composite electrode for the detection of reduced thiolic compounds}}, url = {{http://dx.doi.org/10.1016/j.snb.2007.02.025}}, doi = {{10.1016/j.snb.2007.02.025}}, volume = {{125}}, year = {{2007}}, }