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HvPIP1;6, a barley (Hordeum vulgare L.) plasma membrane water channel particularly expressed in growing compared with non-growing leaf tissues

Wei, Wenxue; Alexandersson, Erik LU ; Golldack, Dortje; Miller, Anthony John; Kjellbom, Per LU and Fricke, Wieland (2007) In Plant and Cell Physiology 48(8). p.1132-1147
Abstract
The aim of the present study was to identify water channel(s) which are expressed specifically in the growth zone of grass leaves and may facilitate growth-associated water uptake into cells. Previously, a gene had been described (HvEmip) which encodes a membrane intrinsic protein (MIP) and which is particularly expressed in the base I cm of barley primary leaves. The functionality of the encoding protein was not known. In the present study on leaf 3 of barley (Hordeum vulgare L.), a clone was isolated, termed HvPIP1;6, which has 99% amino acid sequence identity to HvEmip and belongs to the family of plasma membrane intrinsic proteins (PIPs). Expression of HvPIP1;6 was highest in the elongation zone, where it accounted for > 85% of... (More)
The aim of the present study was to identify water channel(s) which are expressed specifically in the growth zone of grass leaves and may facilitate growth-associated water uptake into cells. Previously, a gene had been described (HvEmip) which encodes a membrane intrinsic protein (MIP) and which is particularly expressed in the base I cm of barley primary leaves. The functionality of the encoding protein was not known. In the present study on leaf 3 of barley (Hordeum vulgare L.), a clone was isolated, termed HvPIP1;6, which has 99% amino acid sequence identity to HvEmip and belongs to the family of plasma membrane intrinsic proteins (PIPs). Expression of HvPIP1;6 was highest in the elongation zone, where it accounted for > 85% of expression of known barley PIP1s. Within the elongation zone, faster grower regions showed higher expression than slower growing regions. Expression of HvPIP1;6 was confined to the epidermis, with some expression in neighboring mesophyll cells. Expression of HvPIP1;6 in Xenopus laevis oocytes increased osmotic water permeability 4- to 6-fold. Water channel activity was inhibited by pre-incubation of oocytes with 50 mu M HgCl2 and increased following incubation with the phosphatase inhibitor okadaic acid or the plant hormone ABA. Plasma membrane preparations were analyzed by Western blots using an antibody that recognized PIP1s. Levels of PIP1s were highest in the elongation and adjacent non-elongation zone. The developmental expression profile of HvPIP2;1, the only known barley water channel belonging to the PIP2 subgroup, was opposite to that of HvPIP1;6. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
membrane intrinsic proteins, growth, leaf, epidermis, barley (Hordeum vulgare L.), cell elongation zone, plasma membrane water channel
in
Plant and Cell Physiology
volume
48
issue
8
pages
1132 - 1147
publisher
Oxford University Press
external identifiers
  • wos:000249497800006
  • scopus:34548008381
ISSN
1471-9053
DOI
10.1093/pcp/pcm083
language
English
LU publication?
yes
id
220b68d5-008a-4b24-8632-7c359c9bcc6e (old id 656992)
date added to LUP
2007-12-19 15:29:46
date last changed
2017-08-13 03:45:52
@article{220b68d5-008a-4b24-8632-7c359c9bcc6e,
  abstract     = {The aim of the present study was to identify water channel(s) which are expressed specifically in the growth zone of grass leaves and may facilitate growth-associated water uptake into cells. Previously, a gene had been described (HvEmip) which encodes a membrane intrinsic protein (MIP) and which is particularly expressed in the base I cm of barley primary leaves. The functionality of the encoding protein was not known. In the present study on leaf 3 of barley (Hordeum vulgare L.), a clone was isolated, termed HvPIP1;6, which has 99% amino acid sequence identity to HvEmip and belongs to the family of plasma membrane intrinsic proteins (PIPs). Expression of HvPIP1;6 was highest in the elongation zone, where it accounted for > 85% of expression of known barley PIP1s. Within the elongation zone, faster grower regions showed higher expression than slower growing regions. Expression of HvPIP1;6 was confined to the epidermis, with some expression in neighboring mesophyll cells. Expression of HvPIP1;6 in Xenopus laevis oocytes increased osmotic water permeability 4- to 6-fold. Water channel activity was inhibited by pre-incubation of oocytes with 50 mu M HgCl2 and increased following incubation with the phosphatase inhibitor okadaic acid or the plant hormone ABA. Plasma membrane preparations were analyzed by Western blots using an antibody that recognized PIP1s. Levels of PIP1s were highest in the elongation and adjacent non-elongation zone. The developmental expression profile of HvPIP2;1, the only known barley water channel belonging to the PIP2 subgroup, was opposite to that of HvPIP1;6.},
  author       = {Wei, Wenxue and Alexandersson, Erik and Golldack, Dortje and Miller, Anthony John and Kjellbom, Per and Fricke, Wieland},
  issn         = {1471-9053},
  keyword      = {membrane intrinsic proteins,growth,leaf,epidermis,barley (Hordeum vulgare L.),cell elongation zone,plasma membrane water channel},
  language     = {eng},
  number       = {8},
  pages        = {1132--1147},
  publisher    = {Oxford University Press},
  series       = {Plant and Cell Physiology},
  title        = {HvPIP1;6, a barley (Hordeum vulgare L.) plasma membrane water channel particularly expressed in growing compared with non-growing leaf tissues},
  url          = {http://dx.doi.org/10.1093/pcp/pcm083},
  volume       = {48},
  year         = {2007},
}