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Interleukin-8 and the neutrophil response to mucosal gram-negative infection

Agace, W. W. LU ; Hedges, S. R. ; Ceska, M. and Svanborg, C. LU (1993) In Journal of Clinical Investigation 92(2). p.780-785
Abstract

Urinary tract infections activate a mucosal inflammatory response, which includes cytokine secretion and neutrophil influx. The mechanisms involved in the neutrophil influx have not been identified. Interleukin-8, a potent chemoattractant for neutrophils, is produced by urinary tract epithelial cell lines in vitro. This study analyzed the human IL-8 response to deliberate Escherichia coli infection of the urinary tract. Urine and serum samples were obtained before and after intravesical instillation of E. coli. Neutrophil numbers were determined on uncentrifuged urine, and IL-8 levels were measured by ELISA. A urinary IL-8 response was found in all patients after bacterial instillation, but no serum IL-8 was detected. There was a strong... (More)

Urinary tract infections activate a mucosal inflammatory response, which includes cytokine secretion and neutrophil influx. The mechanisms involved in the neutrophil influx have not been identified. Interleukin-8, a potent chemoattractant for neutrophils, is produced by urinary tract epithelial cell lines in vitro. This study analyzed the human IL-8 response to deliberate Escherichia coli infection of the urinary tract. Urine and serum samples were obtained before and after intravesical instillation of E. coli. Neutrophil numbers were determined on uncentrifuged urine, and IL-8 levels were measured by ELISA. A urinary IL-8 response was found in all patients after bacterial instillation, but no serum IL-8 was detected. There was a strong correlation between urinary IL-8 levels and urinary neutrophil numbers. The same E. coli strains used to colonize the patients stimulated IL-8 production in urinary tract epithelial cells. The level of IL-8 secreted by epithelial cell lines was influenced by the fimbrial properties of the E. coli. These results demonstrated that E. coli elicit a mucosal IL-8 response in humans, and suggested that IL-8 is involved in the onset of pyuria. Epithelial cells may be an important source of IL-8 during urinary tract infection.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytokines, epithelial cells, Escherichia coli, fimbriae, urinary tract infection
in
Journal of Clinical Investigation
volume
92
issue
2
pages
6 pages
publisher
Am Soc Clin Investig
external identifiers
  • scopus:0027180019
  • pmid:8349817
ISSN
0021-9738
DOI
10.1172/JCI116650
language
English
LU publication?
yes
id
656bfd11-c096-4ac8-b267-97407374b39d
date added to LUP
2019-05-30 14:02:01
date last changed
2020-06-07 06:03:47
@article{656bfd11-c096-4ac8-b267-97407374b39d,
  abstract     = {<p>Urinary tract infections activate a mucosal inflammatory response, which includes cytokine secretion and neutrophil influx. The mechanisms involved in the neutrophil influx have not been identified. Interleukin-8, a potent chemoattractant for neutrophils, is produced by urinary tract epithelial cell lines in vitro. This study analyzed the human IL-8 response to deliberate Escherichia coli infection of the urinary tract. Urine and serum samples were obtained before and after intravesical instillation of E. coli. Neutrophil numbers were determined on uncentrifuged urine, and IL-8 levels were measured by ELISA. A urinary IL-8 response was found in all patients after bacterial instillation, but no serum IL-8 was detected. There was a strong correlation between urinary IL-8 levels and urinary neutrophil numbers. The same E. coli strains used to colonize the patients stimulated IL-8 production in urinary tract epithelial cells. The level of IL-8 secreted by epithelial cell lines was influenced by the fimbrial properties of the E. coli. These results demonstrated that E. coli elicit a mucosal IL-8 response in humans, and suggested that IL-8 is involved in the onset of pyuria. Epithelial cells may be an important source of IL-8 during urinary tract infection.</p>},
  author       = {Agace, W. W. and Hedges, S. R. and Ceska, M. and Svanborg, C.},
  issn         = {0021-9738},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {780--785},
  publisher    = {Am Soc Clin Investig},
  series       = {Journal of Clinical Investigation},
  title        = {Interleukin-8 and the neutrophil response to mucosal gram-negative infection},
  url          = {http://dx.doi.org/10.1172/JCI116650},
  doi          = {10.1172/JCI116650},
  volume       = {92},
  year         = {1993},
}