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Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C-HPP)

Fagerberg, Linn ; Oksvold, Per ; Skogs, Marie ; Algenas, Cajsa ; Lundberg, Emma ; Ponten, Fredrik ; Sivertsson, Asa ; Odeberg, Jacob ; Klevebring, Daniel and Kampf, Caroline , et al. (2013) In Journal of Proteome Research 12(6). p.2439-2448
Abstract
A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few regards to... (More)
A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org). (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antibody-based protein profiling, C-HPP
in
Journal of Proteome Research
volume
12
issue
6
pages
2439 - 2448
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000320298600010
  • scopus:84879327718
  • pmid:23276153
ISSN
1535-3893
DOI
10.1021/pr300924j
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
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6570c309-a1fe-4480-b6c0-5e425f88205b (old id 3990355)
date added to LUP
2016-04-01 11:17:25
date last changed
2020-01-22 02:29:33
@article{6570c309-a1fe-4480-b6c0-5e425f88205b,
  abstract     = {A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).},
  author       = {Fagerberg, Linn and Oksvold, Per and Skogs, Marie and Algenas, Cajsa and Lundberg, Emma and Ponten, Fredrik and Sivertsson, Asa and Odeberg, Jacob and Klevebring, Daniel and Kampf, Caroline and Asplund, Anna and Sjostedt, Evelina and Szigyarto, Cristina Al-Khalili and Edqvist, Per-Henrik and Olsson, IngMarie and Rydberg, Urban and Hudson, Paul and Takanen, Jenny Ottosson and Berling, Holger and Bjorling, Lisa and Tegel, Hanna and Rockberg, Johan and Nilsson, Peter and Navani, Sanjay and Jirström, Karin and Mulder, Jan and Schwenk, Jochen M. and Zwahlen, Martin and Hober, Sophia and Forsberg, Mattias and von Feilitzen, Kalle and Uhlen, Mathias},
  issn         = {1535-3893},
  language     = {eng},
  number       = {6},
  pages        = {2439--2448},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Journal of Proteome Research},
  title        = {Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C-HPP)},
  url          = {http://dx.doi.org/10.1021/pr300924j},
  doi          = {10.1021/pr300924j},
  volume       = {12},
  year         = {2013},
}