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Anaerobic glycerol production by Saccharomyces cerevisiae strains under hyperosmotic stress

Modig, Tobias LU ; Granath, Katarina; Adler, Lennart and Lidén, Gunnar LU (2007) In Applied Microbiology and Biotechnology 75(2). p.289-296
Abstract
Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the combined effect of salt stress and anaerobic conditions, industrial and laboratory strains of S. cerevisiae were grown anaerobically on glucose in batch-cultures containing 40 g/l NaCl. The time needed for complete glucose conversion increased considerably, and the specific growth rates decreased by 80-90% when the cells were subjected to the hyperosmotic conditions. This was accompanied by an increased yield of glycerol and... (More)
Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the combined effect of salt stress and anaerobic conditions, industrial and laboratory strains of S. cerevisiae were grown anaerobically on glucose in batch-cultures containing 40 g/l NaCl. The time needed for complete glucose conversion increased considerably, and the specific growth rates decreased by 80-90% when the cells were subjected to the hyperosmotic conditions. This was accompanied by an increased yield of glycerol and other by-products and reduced biomass yield in all strains. The slowest fermenting strain doubled its glycerol yield (from 0.072 to 0.148 g/g glucose) and a nearly fivefold increase in acetate formation was seen. In more tolerant strains, a lower increase was seen in the glycerol and in the acetate, succinate and pyruvate yields. Additionally, the NADH-producing pathway from acetaldehyde to acetate was analysed by overexpressing the stress-induced gene ALD3. However, this had no or very marginal effect on the acetate and glycerol yields. In the control experiments, the production of NADH from known sources well matched the glycerol formation. This was not the case for the salt stress experiments in which the production of NADH from known sources was insufficient to explain the formed glycerol. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Saccharomyces cerevisiae, osmotic stress, ALD3, glycerol
in
Applied Microbiology and Biotechnology
volume
75
issue
2
pages
289 - 296
publisher
Springer
external identifiers
  • wos:000246842500006
  • scopus:34249883824
ISSN
1432-0614
DOI
10.1007/s00253-006-0821-8
language
English
LU publication?
yes
id
1bfc105e-bb6a-461f-a499-b80cf5254fed (old id 657588)
date added to LUP
2007-12-18 11:20:42
date last changed
2017-07-23 04:46:29
@article{1bfc105e-bb6a-461f-a499-b80cf5254fed,
  abstract     = {Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the combined effect of salt stress and anaerobic conditions, industrial and laboratory strains of S. cerevisiae were grown anaerobically on glucose in batch-cultures containing 40 g/l NaCl. The time needed for complete glucose conversion increased considerably, and the specific growth rates decreased by 80-90% when the cells were subjected to the hyperosmotic conditions. This was accompanied by an increased yield of glycerol and other by-products and reduced biomass yield in all strains. The slowest fermenting strain doubled its glycerol yield (from 0.072 to 0.148 g/g glucose) and a nearly fivefold increase in acetate formation was seen. In more tolerant strains, a lower increase was seen in the glycerol and in the acetate, succinate and pyruvate yields. Additionally, the NADH-producing pathway from acetaldehyde to acetate was analysed by overexpressing the stress-induced gene ALD3. However, this had no or very marginal effect on the acetate and glycerol yields. In the control experiments, the production of NADH from known sources well matched the glycerol formation. This was not the case for the salt stress experiments in which the production of NADH from known sources was insufficient to explain the formed glycerol.},
  author       = {Modig, Tobias and Granath, Katarina and Adler, Lennart and Lidén, Gunnar},
  issn         = {1432-0614},
  keyword      = {Saccharomyces cerevisiae,osmotic stress,ALD3,glycerol},
  language     = {eng},
  number       = {2},
  pages        = {289--296},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Anaerobic glycerol production by Saccharomyces cerevisiae strains under hyperosmotic stress},
  url          = {http://dx.doi.org/10.1007/s00253-006-0821-8},
  volume       = {75},
  year         = {2007},
}