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DNA gel particles: Particle preparation and release characteristics

Moran, M. Carmen; Miguel, M. Graca and Lindman, Björn LU (2007) In Langmuir 23(12). p.6478-6481
Abstract
Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTAB and solutions of the protein lysozyme. Swelling, surface morphology, and DNA release determinations indicate different interaction of ssDNA and dsDNA with both the surfactant and the protein. By using CTAB and lysozyme as the base material, the formation of a DNA reservoir hydrogel, without adding any kind of cross-linker or... (More)
Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTAB and solutions of the protein lysozyme. Swelling, surface morphology, and DNA release determinations indicate different interaction of ssDNA and dsDNA with both the surfactant and the protein. By using CTAB and lysozyme as the base material, the formation of a DNA reservoir hydrogel, without adding any kind of cross-linker or organic solvent, was demostrated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
23
issue
12
pages
6478 - 6481
publisher
The American Chemical Society
external identifiers
  • wos:000246776600002
  • scopus:34250655792
ISSN
0743-7463
DOI
10.1021/la700672e
language
English
LU publication?
yes
id
88c0b78e-f3a3-474d-b017-325afbe77e18 (old id 657765)
date added to LUP
2007-12-18 12:12:50
date last changed
2017-11-19 03:29:27
@article{88c0b78e-f3a3-474d-b017-325afbe77e18,
  abstract     = {Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant CTAB and solutions of the protein lysozyme. Swelling, surface morphology, and DNA release determinations indicate different interaction of ssDNA and dsDNA with both the surfactant and the protein. By using CTAB and lysozyme as the base material, the formation of a DNA reservoir hydrogel, without adding any kind of cross-linker or organic solvent, was demostrated.},
  author       = {Moran, M. Carmen and Miguel, M. Graca and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {12},
  pages        = {6478--6481},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {DNA gel particles: Particle preparation and release characteristics},
  url          = {http://dx.doi.org/10.1021/la700672e},
  volume       = {23},
  year         = {2007},
}