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Paper electrophoretic and immunochemical quantitation of IgG M-components in serum

Grubb, A LU orcid and Skoog, N (1970) In Scandinavian Journal of Clinical and Laboratory Investigation 26(3). p.8-243
Abstract
The IgG M-components of 70 sera were quantitated by a paper electrophoretic method and by an immunochemical method with the use of an antiserum raised against purified polyclonal IgG. The results obtained by the two procedures were concordant and thereby showed that IgG M-components of different antigenic compositions can probably be quantitated by simple immunochemieal methods. Immunochemical determination of the total IgG content of sera containing IgG M-components by the Mancini technique agreed, both when the sera were assessed in their native and in their carbamylated form, with the immunochemical quantitation of Weeke. This indicates that different IgG M-components are immunochemically similarly altered by carbamylation.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Carbamates, Humans, Immune Sera, Immunochemistry, Immunoelectrophoresis, Immunoglobulin M/analysis, Methods
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
26
issue
3
pages
8 - 243
publisher
Informa Healthcare
external identifiers
  • scopus:0014882195
  • pmid:5477097
ISSN
0036-5513
DOI
10.3109/00365517009046229
language
English
LU publication?
yes
id
658ea16c-62c0-4516-865c-413020527c02
date added to LUP
2021-10-16 18:53:55
date last changed
2024-01-12 02:54:35
@article{658ea16c-62c0-4516-865c-413020527c02,
  abstract     = {{The IgG M-components of 70 sera were quantitated by a paper electrophoretic method and by an immunochemical method with the use of an antiserum raised against purified polyclonal IgG. The results obtained by the two procedures were concordant and thereby showed that IgG M-components of different antigenic compositions can probably be quantitated by simple immunochemieal methods. Immunochemical determination of the total IgG content of sera containing IgG M-components by the Mancini technique agreed, both when the sera were assessed in their native and in their carbamylated form, with the immunochemical quantitation of Weeke. This indicates that different IgG M-components are immunochemically similarly altered by carbamylation.}},
  author       = {{Grubb, A and Skoog, N}},
  issn         = {{0036-5513}},
  keywords     = {{Carbamates; Humans; Immune Sera; Immunochemistry; Immunoelectrophoresis; Immunoglobulin M/analysis; Methods}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{8--243}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Paper electrophoretic and immunochemical quantitation of IgG M-components in serum}},
  url          = {{http://dx.doi.org/10.3109/00365517009046229}},
  doi          = {{10.3109/00365517009046229}},
  volume       = {{26}},
  year         = {{1970}},
}