Identification of differentially expressed proteins during human urinary bladder cancer progression
(2005) In Cancer Detection and Prevention 29(3). p.55-249- Abstract
Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss... (More)
Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss of IDPc and Prx-II in more advance tumor samples, in agreement with data on cell lines. These results suggest that loss of IDPc and Prx-II during tumor development may involve in tumor progression and metastasis. However, additional investigations are needed on large number of human samples to further verify these findings.
(Less)
- author
- Memon, Ashfaque A LU ; Chang, Jong W ; Oh, Bong R and Yoo, Yung J
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- keywords
- Blotting, Western, Cell Transformation, Neoplastic, Disease Progression, Down-Regulation, Heat-Shock Proteins/biosynthesis, Humans, Isocitrate Dehydrogenase/biosynthesis, Neoplasm Staging, Peroxidases/biosynthesis, Peroxiredoxins, Tumor Cells, Cultured, Urinary Bladder Neoplasms/genetics
- in
- Cancer Detection and Prevention
- volume
- 29
- issue
- 3
- pages
- 55 - 249
- publisher
- Elsevier
- external identifiers
-
- scopus:20344380665
- pmid:15936593
- ISSN
- 0361-090X
- DOI
- 10.1016/j.cdp.2005.01.002
- language
- English
- LU publication?
- no
- id
- 658f3dfb-706d-441b-a10c-d0900586bdcb
- date added to LUP
- 2019-11-22 16:20:28
- date last changed
- 2024-03-04 08:40:28
@article{658f3dfb-706d-441b-a10c-d0900586bdcb, abstract = {{<p>Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss of IDPc and Prx-II in more advance tumor samples, in agreement with data on cell lines. These results suggest that loss of IDPc and Prx-II during tumor development may involve in tumor progression and metastasis. However, additional investigations are needed on large number of human samples to further verify these findings.</p>}}, author = {{Memon, Ashfaque A and Chang, Jong W and Oh, Bong R and Yoo, Yung J}}, issn = {{0361-090X}}, keywords = {{Blotting, Western; Cell Transformation, Neoplastic; Disease Progression; Down-Regulation; Heat-Shock Proteins/biosynthesis; Humans; Isocitrate Dehydrogenase/biosynthesis; Neoplasm Staging; Peroxidases/biosynthesis; Peroxiredoxins; Tumor Cells, Cultured; Urinary Bladder Neoplasms/genetics}}, language = {{eng}}, number = {{3}}, pages = {{55--249}}, publisher = {{Elsevier}}, series = {{Cancer Detection and Prevention}}, title = {{Identification of differentially expressed proteins during human urinary bladder cancer progression}}, url = {{http://dx.doi.org/10.1016/j.cdp.2005.01.002}}, doi = {{10.1016/j.cdp.2005.01.002}}, volume = {{29}}, year = {{2005}}, }