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Identification of differentially expressed proteins during human urinary bladder cancer progression

Memon, Ashfaque A LU orcid ; Chang, Jong W ; Oh, Bong R and Yoo, Yung J (2005) In Cancer Detection and Prevention 29(3). p.55-249
Abstract

Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss... (More)

Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss of IDPc and Prx-II in more advance tumor samples, in agreement with data on cell lines. These results suggest that loss of IDPc and Prx-II during tumor development may involve in tumor progression and metastasis. However, additional investigations are needed on large number of human samples to further verify these findings.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Blotting, Western, Cell Transformation, Neoplastic, Disease Progression, Down-Regulation, Heat-Shock Proteins/biosynthesis, Humans, Isocitrate Dehydrogenase/biosynthesis, Neoplasm Staging, Peroxidases/biosynthesis, Peroxiredoxins, Tumor Cells, Cultured, Urinary Bladder Neoplasms/genetics
in
Cancer Detection and Prevention
volume
29
issue
3
pages
55 - 249
publisher
Elsevier
external identifiers
  • pmid:15936593
  • scopus:20344380665
ISSN
0361-090X
DOI
10.1016/j.cdp.2005.01.002
language
English
LU publication?
no
id
658f3dfb-706d-441b-a10c-d0900586bdcb
date added to LUP
2019-11-22 16:20:28
date last changed
2024-03-04 08:40:28
@article{658f3dfb-706d-441b-a10c-d0900586bdcb,
  abstract     = {{<p>Comparative proteome analysis was performed between RT4 (grade-1) and T24 (grade-3) bladder cancer cell lines, in an attempt to identify differentially expressed proteins during bladder cancer progression. Among those relatively abundant proteins, seven spots changed more than two-fold reproducibly and identified by peptide mass fingerprinting using mass spectrometry and database search. We found most extensive and reproducible down-regulation of NADP dependent isocitrate dehydrogenase cytoplasmic (IDPc) and peroxiredoxin-II (Prx-II), in poorly differentiated T24 compared to well-differentiated RT4 bladder cancer cell line. Subsequent Western blotting analysis of human biopsy samples from bladder cancer patient revealed significant loss of IDPc and Prx-II in more advance tumor samples, in agreement with data on cell lines. These results suggest that loss of IDPc and Prx-II during tumor development may involve in tumor progression and metastasis. However, additional investigations are needed on large number of human samples to further verify these findings.</p>}},
  author       = {{Memon, Ashfaque A and Chang, Jong W and Oh, Bong R and Yoo, Yung J}},
  issn         = {{0361-090X}},
  keywords     = {{Blotting, Western; Cell Transformation, Neoplastic; Disease Progression; Down-Regulation; Heat-Shock Proteins/biosynthesis; Humans; Isocitrate Dehydrogenase/biosynthesis; Neoplasm Staging; Peroxidases/biosynthesis; Peroxiredoxins; Tumor Cells, Cultured; Urinary Bladder Neoplasms/genetics}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{55--249}},
  publisher    = {{Elsevier}},
  series       = {{Cancer Detection and Prevention}},
  title        = {{Identification of differentially expressed proteins during human urinary bladder cancer progression}},
  url          = {{http://dx.doi.org/10.1016/j.cdp.2005.01.002}},
  doi          = {{10.1016/j.cdp.2005.01.002}},
  volume       = {{29}},
  year         = {{2005}},
}