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Genetic diversity in hepatitis C virus in Egypt and possible association with hepatocellular carcinoma

Abdel-Hamid, Mohamed; El-Daly, Mai; Molnegren, Vilma; El-Kafrawy, Sherif; Abdel-Latif, Sohair; Esmat, Gamal; Strickland, G. Thomas; Loffredo, Christopher; Albert, Jan and Widell, Anders LU (2007) In Journal of General Virology 88(5). p.1526-1531
Abstract
Egypt has one of the world's highest prevalences of hepatitis C virus (HCV) infection, with a majority of genotype 4 infections. To explore the genetic diversity of HCV in Egypt, sera from 131 Egyptians [56 from community studies, 37 chronic hepatitis patients, 28 hepatocellular carcinoma (HCC) patients and 10 patients with non-Hodgkin's lymphoma] were genotyped by restriction fragment-length polymorphism and phylogenetic analyses of sequences from the mid-core and non-structural 5B regions. The different genotyping methods showed good agreement. The majority of the viruses (83 of 131; 63 %) were of subtype 4a, but five other subtypes within genotype 4 were also observed, as well as three genotype 1b, five genotype 1g and one genotype 3a... (More)
Egypt has one of the world's highest prevalences of hepatitis C virus (HCV) infection, with a majority of genotype 4 infections. To explore the genetic diversity of HCV in Egypt, sera from 131 Egyptians [56 from community studies, 37 chronic hepatitis patients, 28 hepatocellular carcinoma (HCC) patients and 10 patients with non-Hodgkin's lymphoma] were genotyped by restriction fragment-length polymorphism and phylogenetic analyses of sequences from the mid-core and non-structural 5B regions. The different genotyping methods showed good agreement. The majority of the viruses (83 of 131; 63 %) were of subtype 4a, but five other subtypes within genotype 4 were also observed, as well as three genotype 1b, five genotype 1g and one genotype 3a samples. Interestingly, subtype 4o, which was easily identifiable in all three genomic regions, 3 showed an association with HCC (P=0.017), which merits further investigation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
88
issue
5
pages
1526 - 1531
publisher
Society for General Microbiology
external identifiers
  • wos:000246532300017
  • scopus:34248220080
ISSN
1465-2099
DOI
10.1099/vir.0.82626-0
language
English
LU publication?
yes
id
abe57d10-29e8-4a03-9f11-d0de0c62b087 (old id 659421)
date added to LUP
2007-12-11 08:57:21
date last changed
2017-11-12 04:00:54
@article{abe57d10-29e8-4a03-9f11-d0de0c62b087,
  abstract     = {Egypt has one of the world's highest prevalences of hepatitis C virus (HCV) infection, with a majority of genotype 4 infections. To explore the genetic diversity of HCV in Egypt, sera from 131 Egyptians [56 from community studies, 37 chronic hepatitis patients, 28 hepatocellular carcinoma (HCC) patients and 10 patients with non-Hodgkin's lymphoma] were genotyped by restriction fragment-length polymorphism and phylogenetic analyses of sequences from the mid-core and non-structural 5B regions. The different genotyping methods showed good agreement. The majority of the viruses (83 of 131; 63 %) were of subtype 4a, but five other subtypes within genotype 4 were also observed, as well as three genotype 1b, five genotype 1g and one genotype 3a samples. Interestingly, subtype 4o, which was easily identifiable in all three genomic regions, 3 showed an association with HCC (P=0.017), which merits further investigation.},
  author       = {Abdel-Hamid, Mohamed and El-Daly, Mai and Molnegren, Vilma and El-Kafrawy, Sherif and Abdel-Latif, Sohair and Esmat, Gamal and Strickland, G. Thomas and Loffredo, Christopher and Albert, Jan and Widell, Anders},
  issn         = {1465-2099},
  language     = {eng},
  number       = {5},
  pages        = {1526--1531},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {Genetic diversity in hepatitis C virus in Egypt and possible association with hepatocellular carcinoma},
  url          = {http://dx.doi.org/10.1099/vir.0.82626-0},
  volume       = {88},
  year         = {2007},
}