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Carbon ceramic electrodes modified with laccase from Trametes hirsuta: Fabrication, characterization and their use for phenolic compounds detection

Haghighi, Behzad; Rahmati-Panah, Amir; Shleev, Sergey LU and Gorton, Lo LU (2007) In Electroanalysis 19(9). p.907-917
Abstract
Fungal laccase (Lc) from the basidiomycete Trametes hirsuta was immobilized on top of a carbon ceramic electrode using physical absorption. Direct, unmediated heterogeneous electron transfer between Lc and the carbon ceramic electrode (CCE) under aerobic conditions was shown. The bioelectrocatalytic reduction of oxygen on Lc-CCE started at about 430 mV vs. Ag vertical bar AgCl vertical bar KClsat at pH 3.5 and moved with about 57 mV in the cathodic region per pH unit. The Lc-modified CCE was then used as a biosensing detection element in a single line flow injection system for the amperometric determination of a variety of phenolic substrates of the enzyme. The experimental conditions were studied and optimized for catechol serving as a... (More)
Fungal laccase (Lc) from the basidiomycete Trametes hirsuta was immobilized on top of a carbon ceramic electrode using physical absorption. Direct, unmediated heterogeneous electron transfer between Lc and the carbon ceramic electrode (CCE) under aerobic conditions was shown. The bioelectrocatalytic reduction of oxygen on Lc-CCE started at about 430 mV vs. Ag vertical bar AgCl vertical bar KClsat at pH 3.5 and moved with about 57 mV in the cathodic region per pH unit. The Lc-modified CCE was then used as a biosensing detection element in a single line flow injection system for the amperometric determination of a variety of phenolic substrates of the enzyme. The experimental conditions were studied and optimized for catechol serving as a model compound. Statistical aspects were applied and the sensor characteristics and Michaelis-Menten constants of the investigated phenolic compounds were calculated and compared with those obtained for solid graphite electrodes modified with Trametes hirsuta laccase. The results showed that the CCE based biosensor in comparison with the solid graphite based biosensor offers a lower detection limit, a wider linear dynamic range, and excellent operational stability with no sensor passivation, indicating that the sol - gel lattice improves the electrochemical behavior of the biosensor. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
electrochemical, carbon ceramic electrode, phenol, biosensor, laccase, behavior
in
Electroanalysis
volume
19
issue
9
pages
907 - 917
publisher
John Wiley & Sons
external identifiers
  • wos:000246534700001
  • scopus:34547584295
ISSN
1040-0397
DOI
10.1002/elan.200603839
language
English
LU publication?
yes
id
ecb58a4b-8011-40a9-af75-82d940bc596a (old id 659639)
date added to LUP
2007-12-07 14:41:21
date last changed
2017-04-16 04:15:36
@article{ecb58a4b-8011-40a9-af75-82d940bc596a,
  abstract     = {Fungal laccase (Lc) from the basidiomycete Trametes hirsuta was immobilized on top of a carbon ceramic electrode using physical absorption. Direct, unmediated heterogeneous electron transfer between Lc and the carbon ceramic electrode (CCE) under aerobic conditions was shown. The bioelectrocatalytic reduction of oxygen on Lc-CCE started at about 430 mV vs. Ag vertical bar AgCl vertical bar KClsat at pH 3.5 and moved with about 57 mV in the cathodic region per pH unit. The Lc-modified CCE was then used as a biosensing detection element in a single line flow injection system for the amperometric determination of a variety of phenolic substrates of the enzyme. The experimental conditions were studied and optimized for catechol serving as a model compound. Statistical aspects were applied and the sensor characteristics and Michaelis-Menten constants of the investigated phenolic compounds were calculated and compared with those obtained for solid graphite electrodes modified with Trametes hirsuta laccase. The results showed that the CCE based biosensor in comparison with the solid graphite based biosensor offers a lower detection limit, a wider linear dynamic range, and excellent operational stability with no sensor passivation, indicating that the sol - gel lattice improves the electrochemical behavior of the biosensor.},
  author       = {Haghighi, Behzad and Rahmati-Panah, Amir and Shleev, Sergey and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {electrochemical,carbon ceramic electrode,phenol,biosensor,laccase,behavior},
  language     = {eng},
  number       = {9},
  pages        = {907--917},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Carbon ceramic electrodes modified with laccase from Trametes hirsuta: Fabrication, characterization and their use for phenolic compounds detection},
  url          = {http://dx.doi.org/10.1002/elan.200603839},
  volume       = {19},
  year         = {2007},
}