Solvent-free enzymatic synthesis of fatty alkanolamides
(2007) In Biotechnology and Bioengineering 97(3). p.447-453- Abstract
- An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions... (More)
- An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions to avoid co-distillation of the amine were required, Two different stratagies to avoid the amine loss are presented. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/659702
- author
- Tufvesson, Pär LU ; Annerling, Annika ; Hatti-Kaul, Rajni LU and Adlercreutz, Dietlind LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immobilized lipase, ethanolamine, amide, biosurfactant
- in
- Biotechnology and Bioengineering
- volume
- 97
- issue
- 3
- pages
- 447 - 453
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000246491700002
- scopus:34249812929
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.21258
- language
- English
- LU publication?
- yes
- id
- b4e61ac2-0dc7-44de-8cb5-307c8c83b98f (old id 659702)
- date added to LUP
- 2016-04-01 11:40:26
- date last changed
- 2022-01-26 08:33:44
@article{b4e61ac2-0dc7-44de-8cb5-307c8c83b98f,
abstract = {{An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions to avoid co-distillation of the amine were required, Two different stratagies to avoid the amine loss are presented.}},
author = {{Tufvesson, Pär and Annerling, Annika and Hatti-Kaul, Rajni and Adlercreutz, Dietlind}},
issn = {{1097-0290}},
keywords = {{immobilized lipase; ethanolamine; amide; biosurfactant}},
language = {{eng}},
number = {{3}},
pages = {{447--453}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Solvent-free enzymatic synthesis of fatty alkanolamides}},
url = {{http://dx.doi.org/10.1002/bit.21258}},
doi = {{10.1002/bit.21258}},
volume = {{97}},
year = {{2007}},
}