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Solvent-free enzymatic synthesis of fatty alkanolamides

Tufvesson, Pär LU ; Annerling, Annika; Hatti-Kaul, Rajni LU and Adlercreutz, Dietlind LU (2007) In Biotechnology and Bioengineering 97(3). p.447-453
Abstract
An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions... (More)
An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions to avoid co-distillation of the amine were required, Two different stratagies to avoid the amine loss are presented. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immobilized lipase, ethanolamine, amide, biosurfactant
in
Biotechnology and Bioengineering
volume
97
issue
3
pages
447 - 453
publisher
John Wiley & Sons
external identifiers
  • wos:000246491700002
  • scopus:34249812929
ISSN
1097-0290
DOI
10.1002/bit.21258
language
English
LU publication?
yes
id
b4e61ac2-0dc7-44de-8cb5-307c8c83b98f (old id 659702)
date added to LUP
2007-12-20 10:42:39
date last changed
2017-10-22 03:35:17
@article{b4e61ac2-0dc7-44de-8cb5-307c8c83b98f,
  abstract     = {An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized Candida antarctica lipase B, Novozym (R) 435, was used to catalyze the condensation of lauric acid with monothanolamine. The raction temperature of 90 degrees C was required to keep the reactants in a liquid state. Atepwise addition of the amine minimized problems caused by the formation of a highly viscous amine/fatty acid ion pair. The enzyme was both very active and stable under the reaction conditions, with about half of the activity remaining after 2 weeks. The maximum amide yield obtained when using equimolar amounts of the r4eaction wa 75%, which could be increased to 95% upon water removal. Special precautions to avoid co-distillation of the amine were required, Two different stratagies to avoid the amine loss are presented.},
  author       = {Tufvesson, Pär and Annerling, Annika and Hatti-Kaul, Rajni and Adlercreutz, Dietlind},
  issn         = {1097-0290},
  keyword      = {immobilized lipase,ethanolamine,amide,biosurfactant},
  language     = {eng},
  number       = {3},
  pages        = {447--453},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Solvent-free enzymatic synthesis of fatty alkanolamides},
  url          = {http://dx.doi.org/10.1002/bit.21258},
  volume       = {97},
  year         = {2007},
}