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Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

Volpati, Diogo LU ; Aoki, Pedro H.B. ; Alessio, Priscila ; Pavinatto, Felippe J. ; Miranda, Paulo B. ; Constantino, Carlos J.L. and Oliveira, Osvaldo N. (2014) In Advances in Colloid and Interface Science 207(1). p.199-215
Abstract

Investigation into nanostructured organic films has served many purposes, including the design of functionalized surfaces that may be applied in biomedical devices and tissue engineering and for studying physiological processes depending on the interaction with cell membranes. Of particular relevance are Langmuir monolayers, Langmuir-Blodgett (LB) and layer-by-layer (LbL) films used to simulate biological interfaces. In this review, we shall focus on the use of vibrational spectroscopy methods to probe molecular-level interactions at biomimetic interfaces, with special emphasis on three surface-specific techniques, namely sum frequency generation (SFG), polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and... (More)

Investigation into nanostructured organic films has served many purposes, including the design of functionalized surfaces that may be applied in biomedical devices and tissue engineering and for studying physiological processes depending on the interaction with cell membranes. Of particular relevance are Langmuir monolayers, Langmuir-Blodgett (LB) and layer-by-layer (LbL) films used to simulate biological interfaces. In this review, we shall focus on the use of vibrational spectroscopy methods to probe molecular-level interactions at biomimetic interfaces, with special emphasis on three surface-specific techniques, namely sum frequency generation (SFG), polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and surface-enhanced Raman scattering (SERS). The two types of systems selected for exemplifying the potential of the methods are the cell membrane models and the functionalized surfaces with biomolecules. Examples will be given on how SFG and PM-IRRAS can be combined to determine the effects from biomolecules on cell membrane models, which include determination of the orientation and preservation of secondary structure. Crucial information for the action of biomolecules on model membranes has also been obtained with PM-IRRAS, as is the case of chitosan removing proteins from the membrane. SERS will be shown as promising for enabling detection limits down to the single-molecule level. The strengths and limitations of these methods will also be discussed, in addition to the prospects for the near future.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Biointerfaces, Biomolecules, Cell-membrane models, Surface functionalization, Thin nanostructured films, Vibrational spectroscopy
in
Advances in Colloid and Interface Science
volume
207
issue
1
pages
17 pages
publisher
Elsevier
external identifiers
  • pmid:24530000
  • scopus:84901187939
ISSN
0001-8686
DOI
10.1016/j.cis.2014.01.014
language
English
LU publication?
no
id
65a09876-8eb8-4510-9d92-502e4865cd7e
date added to LUP
2019-05-17 14:35:15
date last changed
2021-01-12 02:25:12
@article{65a09876-8eb8-4510-9d92-502e4865cd7e,
  abstract     = {<p>Investigation into nanostructured organic films has served many purposes, including the design of functionalized surfaces that may be applied in biomedical devices and tissue engineering and for studying physiological processes depending on the interaction with cell membranes. Of particular relevance are Langmuir monolayers, Langmuir-Blodgett (LB) and layer-by-layer (LbL) films used to simulate biological interfaces. In this review, we shall focus on the use of vibrational spectroscopy methods to probe molecular-level interactions at biomimetic interfaces, with special emphasis on three surface-specific techniques, namely sum frequency generation (SFG), polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and surface-enhanced Raman scattering (SERS). The two types of systems selected for exemplifying the potential of the methods are the cell membrane models and the functionalized surfaces with biomolecules. Examples will be given on how SFG and PM-IRRAS can be combined to determine the effects from biomolecules on cell membrane models, which include determination of the orientation and preservation of secondary structure. Crucial information for the action of biomolecules on model membranes has also been obtained with PM-IRRAS, as is the case of chitosan removing proteins from the membrane. SERS will be shown as promising for enabling detection limits down to the single-molecule level. The strengths and limitations of these methods will also be discussed, in addition to the prospects for the near future.</p>},
  author       = {Volpati, Diogo and Aoki, Pedro H.B. and Alessio, Priscila and Pavinatto, Felippe J. and Miranda, Paulo B. and Constantino, Carlos J.L. and Oliveira, Osvaldo N.},
  issn         = {0001-8686},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {199--215},
  publisher    = {Elsevier},
  series       = {Advances in Colloid and Interface Science},
  title        = {Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces},
  url          = {http://dx.doi.org/10.1016/j.cis.2014.01.014},
  doi          = {10.1016/j.cis.2014.01.014},
  volume       = {207},
  year         = {2014},
}