Advanced

Buffer formulation affects the interaction between lysozyme and polymeric nanoparticles

Lundqvist, Martin LU and Cabaleiro-Lago, Celia LU (2017) In Journal of Colloid and Interface Science 504. p.78-85
Abstract

The effect of the buffer formulation in terms of buffer identity and ionic strength on the interaction between chicken egg lysozyme and carboxyl-modified polystyrene nanoparticles has been systematically studied. The time evolution of the fluorescence of a reporter molecule shows that lysozyme interacts with the nanoparticles in all the studied conditions. The interaction results in changes in protein conformation and decrease of the colloidal stability of nanoparticles. In absence of a background salt the rate of adsorption is affected mainly by the ionic strength of the buffer solution, although, specific buffer effects may contribute to a certain extent. The identity of the different buffer components does not significantly alter the... (More)

The effect of the buffer formulation in terms of buffer identity and ionic strength on the interaction between chicken egg lysozyme and carboxyl-modified polystyrene nanoparticles has been systematically studied. The time evolution of the fluorescence of a reporter molecule shows that lysozyme interacts with the nanoparticles in all the studied conditions. The interaction results in changes in protein conformation and decrease of the colloidal stability of nanoparticles. In absence of a background salt the rate of adsorption is affected mainly by the ionic strength of the buffer solution, although, specific buffer effects may contribute to a certain extent. The identity of the different buffer components does not significantly alter the dynamics of the process in presence of salt at constant ionic strength. However, an increase of ionic strength leads to slower processes indicating that the adsorption is affected by the presence of increasing number of ions in solution.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Buffer, Dynamics, Interaction, Ionic strength, Nanoparticles, Protein, Zeta-potential
in
Journal of Colloid and Interface Science
volume
504
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85019500161
  • wos:000405646200008
ISSN
0021-9797
DOI
10.1016/j.jcis.2017.05.031
language
English
LU publication?
yes
id
65ac0541-5047-40d0-9b82-aa0fac4eb31e
date added to LUP
2017-06-08 09:55:19
date last changed
2017-09-18 11:39:19
@article{65ac0541-5047-40d0-9b82-aa0fac4eb31e,
  abstract     = {<p>The effect of the buffer formulation in terms of buffer identity and ionic strength on the interaction between chicken egg lysozyme and carboxyl-modified polystyrene nanoparticles has been systematically studied. The time evolution of the fluorescence of a reporter molecule shows that lysozyme interacts with the nanoparticles in all the studied conditions. The interaction results in changes in protein conformation and decrease of the colloidal stability of nanoparticles. In absence of a background salt the rate of adsorption is affected mainly by the ionic strength of the buffer solution, although, specific buffer effects may contribute to a certain extent. The identity of the different buffer components does not significantly alter the dynamics of the process in presence of salt at constant ionic strength. However, an increase of ionic strength leads to slower processes indicating that the adsorption is affected by the presence of increasing number of ions in solution.</p>},
  author       = {Lundqvist, Martin and Cabaleiro-Lago, Celia},
  issn         = {0021-9797},
  keyword      = {Buffer,Dynamics,Interaction,Ionic strength,Nanoparticles,Protein,Zeta-potential},
  language     = {eng},
  month        = {10},
  pages        = {78--85},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Buffer formulation affects the interaction between lysozyme and polymeric nanoparticles},
  url          = {http://dx.doi.org/10.1016/j.jcis.2017.05.031},
  volume       = {504},
  year         = {2017},
}