Galectin-3 guides intracellular trafficking of some human serotransferrin glycoforms
(2013) In Journal of Biological Chemistry 288(39). p.28398-28408- Abstract
- Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a... (More)
- Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a functional role for transferrin glycans, in intracellular trafficking after uptake. Moreover, the galectin-3 bound glycoform increased in cancer, suggesting a pathophysiological regulation. These are novel aspects of transferrin cell biology, which has previously considered only degree of iron loading, but not other forms of heterogeneity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965293
- author
- Carlsson, Michael LU ; Bengtson, Per LU ; Cucak, Helena LU and Leffler, Hakon LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Galectin, transferrin, glycoforms, trafficking, endocytosis
- in
- Journal of Biological Chemistry
- volume
- 288
- issue
- 39
- pages
- 28398 - 28408
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:84884807164
- pmid:23926108
- wos:000330612800067
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M113.487793
- language
- English
- LU publication?
- yes
- id
- 65eef5f5-96bf-45c3-81b5-03126f2d2c34 (old id 3965293)
- alternative location
- http://www.jbc.org/content/early/2013/08/07/jbc.M113.487793.abstract
- date added to LUP
- 2016-04-04 07:36:35
- date last changed
- 2022-04-23 08:29:09
@article{65eef5f5-96bf-45c3-81b5-03126f2d2c34,
abstract = {{Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a functional role for transferrin glycans, in intracellular trafficking after uptake. Moreover, the galectin-3 bound glycoform increased in cancer, suggesting a pathophysiological regulation. These are novel aspects of transferrin cell biology, which has previously considered only degree of iron loading, but not other forms of heterogeneity.}},
author = {{Carlsson, Michael and Bengtson, Per and Cucak, Helena and Leffler, Hakon}},
issn = {{1083-351X}},
keywords = {{Galectin; transferrin; glycoforms; trafficking; endocytosis}},
language = {{eng}},
number = {{39}},
pages = {{28398--28408}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Biological Chemistry}},
title = {{Galectin-3 guides intracellular trafficking of some human serotransferrin glycoforms}},
url = {{http://dx.doi.org/10.1074/jbc.M113.487793}},
doi = {{10.1074/jbc.M113.487793}},
volume = {{288}},
year = {{2013}},
}