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Galectin-3 guides intracellular trafficking of some human serotransferrin glycoforms

Carlsson, Michael LU ; Bengtson, Per LU ; Cucak, Helena LU and Leffler, Hakon LU (2013) In Journal of Biological Chemistry 288(39). p.28398-28408
Abstract
Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a... (More)
Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a functional role for transferrin glycans, in intracellular trafficking after uptake. Moreover, the galectin-3 bound glycoform increased in cancer, suggesting a pathophysiological regulation. These are novel aspects of transferrin cell biology, which has previously considered only degree of iron loading, but not other forms of heterogeneity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Galectin, transferrin, glycoforms, trafficking, endocytosis
in
Journal of Biological Chemistry
volume
288
issue
39
pages
28398 - 28408
publisher
ASBMB
external identifiers
  • scopus:84884807164
  • wos:000330612800067
ISSN
1083-351X
DOI
10.1074/jbc.M113.487793
language
English
LU publication?
yes
id
65eef5f5-96bf-45c3-81b5-03126f2d2c34 (old id 3965293)
alternative location
http://www.jbc.org/content/early/2013/08/07/jbc.M113.487793.abstract
date added to LUP
2016-01-28 14:19:34
date last changed
2019-03-10 04:14:39
@article{65eef5f5-96bf-45c3-81b5-03126f2d2c34,
  abstract     = {Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a functional role for transferrin glycans, in intracellular trafficking after uptake. Moreover, the galectin-3 bound glycoform increased in cancer, suggesting a pathophysiological regulation. These are novel aspects of transferrin cell biology, which has previously considered only degree of iron loading, but not other forms of heterogeneity.},
  author       = {Carlsson, Michael and Bengtson, Per and Cucak, Helena and Leffler, Hakon},
  issn         = {1083-351X},
  keyword      = {Galectin,transferrin,glycoforms,trafficking,endocytosis},
  language     = {eng},
  number       = {39},
  pages        = {28398--28408},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Galectin-3 guides intracellular trafficking of some human serotransferrin glycoforms},
  url          = {http://dx.doi.org/10.1074/jbc.M113.487793},
  volume       = {288},
  year         = {2013},
}