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Mode of action of acidocin D20079, a bacteriocin produced by the potential probiotic strain, Lactobacillus acidophilus DSM 20079

Deraz, Sahar LU ; Nordberg Karlsson, Eva LU ; Khalil, Ashraf A. and Mattiasson, Bo LU (2007) In Journal of Industrial Microbiology & Biotechnology 34(5). p.373-379
Abstract
Lactobacillus acidophilus DSM 20079 is the producer of a novel bacteriocin termed acidocin D20079. In this paper, mode of action using three various concentrations of acidocin D20079 (2,048, 128 and 11.3 AU/ml) was determined against an indicator strain L. delbrueckii subsp. lactis DSM 20076. These concentrations all led to marked decreases in both the number of viable cells and in optical density, indicating that the activity of the acidocin D20079 was bactericidal with concomitant cell lysis. Moreover, the probiotic potential of L. acidophilus DSM 20079 was analyzed for its ability to survive and retain viability at conditions (acid and bile concentrations) mimicking the gastrointestinal (GI) tract, under which it survived exposure to pH... (More)
Lactobacillus acidophilus DSM 20079 is the producer of a novel bacteriocin termed acidocin D20079. In this paper, mode of action using three various concentrations of acidocin D20079 (2,048, 128 and 11.3 AU/ml) was determined against an indicator strain L. delbrueckii subsp. lactis DSM 20076. These concentrations all led to marked decreases in both the number of viable cells and in optical density, indicating that the activity of the acidocin D20079 was bactericidal with concomitant cell lysis. Moreover, the probiotic potential of L. acidophilus DSM 20079 was analyzed for its ability to survive and retain viability at conditions (acid and bile concentrations) mimicking the gastrointestinal (GI) tract, under which it survived exposure to pH 2.0 with a 1.2 log cycle reduction in viability and where 45% of the original population survived in a medium containing 0.3% bile for 3 h. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antimicrobial protein, bacteriocin, acidocin D20079, Lactobacillu, inhibitory activity, probiotic
in
Journal of Industrial Microbiology & Biotechnology
volume
34
issue
5
pages
373 - 379
publisher
Springer
external identifiers
  • wos:000246303700005
  • scopus:34247173125
ISSN
1367-5435
DOI
10.1007/s10295-007-0206-8
language
English
LU publication?
yes
id
82715213-81de-42e3-be6d-909551007102 (old id 660459)
date added to LUP
2007-12-11 11:36:20
date last changed
2017-01-01 07:23:09
@article{82715213-81de-42e3-be6d-909551007102,
  abstract     = {Lactobacillus acidophilus DSM 20079 is the producer of a novel bacteriocin termed acidocin D20079. In this paper, mode of action using three various concentrations of acidocin D20079 (2,048, 128 and 11.3 AU/ml) was determined against an indicator strain L. delbrueckii subsp. lactis DSM 20076. These concentrations all led to marked decreases in both the number of viable cells and in optical density, indicating that the activity of the acidocin D20079 was bactericidal with concomitant cell lysis. Moreover, the probiotic potential of L. acidophilus DSM 20079 was analyzed for its ability to survive and retain viability at conditions (acid and bile concentrations) mimicking the gastrointestinal (GI) tract, under which it survived exposure to pH 2.0 with a 1.2 log cycle reduction in viability and where 45% of the original population survived in a medium containing 0.3% bile for 3 h.},
  author       = {Deraz, Sahar and Nordberg Karlsson, Eva and Khalil, Ashraf A. and Mattiasson, Bo},
  issn         = {1367-5435},
  keyword      = {antimicrobial protein,bacteriocin,acidocin D20079,Lactobacillu,inhibitory activity,probiotic},
  language     = {eng},
  number       = {5},
  pages        = {373--379},
  publisher    = {Springer},
  series       = {Journal of Industrial Microbiology & Biotechnology},
  title        = {Mode of action of acidocin D20079, a bacteriocin produced by the potential probiotic strain, Lactobacillus acidophilus DSM 20079},
  url          = {http://dx.doi.org/10.1007/s10295-007-0206-8},
  volume       = {34},
  year         = {2007},
}