Advanced

Globin-expression postpones onset of stationary phase specific gene expression in Escherichia coli

Frey, Alexander D.; Andersson, Charlotte LU ; Schmid, Vinzenz H.; Bülow, Leif LU and Kallio, Pauli T. (2007) In Journal of Biotechnology 129(3). p.461-471
Abstract
We have analyzed gene expression of Escherichia coli MG1655 expressing native and engineered bacterial globin proteins, in order to identify the molecular mechanisms leading to the improved phenotypical traits relative to control cells under oxygen-limited conditions. Regulated expression of hemoglobin and flavohemoglobin proteins postponed the onset of rpoS expression relative to plasmid bearing control cells. This change in expression pattern coincided with the expression pattern of stationary-phase specific genes including sigma(S)-dependent and sigma(S)-independent genes. Furthermore, several genes known to affect rpoS transcription, rpoS mRNA stability and sigma(S) turnover were regulated in such a manner as to ultimately lower the... (More)
We have analyzed gene expression of Escherichia coli MG1655 expressing native and engineered bacterial globin proteins, in order to identify the molecular mechanisms leading to the improved phenotypical traits relative to control cells under oxygen-limited conditions. Regulated expression of hemoglobin and flavohemoglobin proteins postponed the onset of rpoS expression relative to plasmid bearing control cells. This change in expression pattern coincided with the expression pattern of stationary-phase specific genes including sigma(S)-dependent and sigma(S)-independent genes. Furthermore, several genes known to affect rpoS transcription, rpoS mRNA stability and sigma(S) turnover were regulated in such a manner as to ultimately lower the cellular level of sigma(S) in all globin-expressing strains. In a strain harboring an rpoS-lacZ fusion, lacZ expression correlated with acetate accumulation, a metabolite that is known to activate rpoS transcription, but not with growth. Therefore, we hypothesize that reduced excretion of acetate in globin expressing cells prevents induction of stationary phase specific genes. Additionally, several genes responding to carbon starvation (e.g. csrAB, cstA, sspA) were expressed at lower levels in globin-expressing cells. These findings are in good agreement with previous reports showing a more efficient energy household, i.e. also reduced glucose consumption, in hemoglobin- and flavohemoglobin-expressing cells relative to controls. (c) 2007 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ralstonia eutropha flavohemoprotein, oxygen-limited growth, Vitreoscilla hemoglobin, expression profiling, stationary phase
in
Journal of Biotechnology
volume
129
issue
3
pages
461 - 471
publisher
Elsevier
external identifiers
  • wos:000246350600013
  • scopus:34147097589
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2007.01.012
language
English
LU publication?
yes
id
dd05661e-58ac-4433-972a-7cd4a8261a13 (old id 660468)
date added to LUP
2007-12-10 14:20:02
date last changed
2017-01-01 04:37:49
@article{dd05661e-58ac-4433-972a-7cd4a8261a13,
  abstract     = {We have analyzed gene expression of Escherichia coli MG1655 expressing native and engineered bacterial globin proteins, in order to identify the molecular mechanisms leading to the improved phenotypical traits relative to control cells under oxygen-limited conditions. Regulated expression of hemoglobin and flavohemoglobin proteins postponed the onset of rpoS expression relative to plasmid bearing control cells. This change in expression pattern coincided with the expression pattern of stationary-phase specific genes including sigma(S)-dependent and sigma(S)-independent genes. Furthermore, several genes known to affect rpoS transcription, rpoS mRNA stability and sigma(S) turnover were regulated in such a manner as to ultimately lower the cellular level of sigma(S) in all globin-expressing strains. In a strain harboring an rpoS-lacZ fusion, lacZ expression correlated with acetate accumulation, a metabolite that is known to activate rpoS transcription, but not with growth. Therefore, we hypothesize that reduced excretion of acetate in globin expressing cells prevents induction of stationary phase specific genes. Additionally, several genes responding to carbon starvation (e.g. csrAB, cstA, sspA) were expressed at lower levels in globin-expressing cells. These findings are in good agreement with previous reports showing a more efficient energy household, i.e. also reduced glucose consumption, in hemoglobin- and flavohemoglobin-expressing cells relative to controls. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Frey, Alexander D. and Andersson, Charlotte and Schmid, Vinzenz H. and Bülow, Leif and Kallio, Pauli T.},
  issn         = {1873-4863},
  keyword      = {Ralstonia eutropha flavohemoprotein,oxygen-limited growth,Vitreoscilla hemoglobin,expression profiling,stationary phase},
  language     = {eng},
  number       = {3},
  pages        = {461--471},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Globin-expression postpones onset of stationary phase specific gene expression in Escherichia coli},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2007.01.012},
  volume       = {129},
  year         = {2007},
}