Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages
(2007) In Biosensors & Bioelectronics 22(11). p.2611-2617- Abstract
- This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good... (More)
- This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/660590
- author
- Antiochia, Riccarda and Gorton, Lo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- osmium redox, diaphorase, glucose dehydrogenase, biosensor, glucose, polymer, carbon nanotube paste electrode
- in
- Biosensors & Bioelectronics
- volume
- 22
- issue
- 11
- pages
- 2611 - 2617
- publisher
- Elsevier
- external identifiers
-
- wos:000246382500031
- scopus:33947584425
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2006.10.023
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- f635f056-535c-4538-87cd-f9b939bab7c7 (old id 660590)
- date added to LUP
- 2016-04-01 16:24:55
- date last changed
- 2022-03-15 00:23:43
@article{f635f056-535c-4538-87cd-f9b939bab7c7, abstract = {{This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved.}}, author = {{Antiochia, Riccarda and Gorton, Lo}}, issn = {{1873-4235}}, keywords = {{osmium redox; diaphorase; glucose dehydrogenase; biosensor; glucose; polymer; carbon nanotube paste electrode}}, language = {{eng}}, number = {{11}}, pages = {{2611--2617}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages}}, url = {{http://dx.doi.org/10.1016/j.bios.2006.10.023}}, doi = {{10.1016/j.bios.2006.10.023}}, volume = {{22}}, year = {{2007}}, }