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Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages

Antiochia, Riccarda and Gorton, Lo LU (2007) In Biosensors & Bioelectronics 22(11). p.2611-2617
Abstract
This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good... (More)
This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
osmium redox, diaphorase, glucose dehydrogenase, biosensor, glucose, polymer, carbon nanotube paste electrode
in
Biosensors & Bioelectronics
volume
22
issue
11
pages
2611 - 2617
publisher
Elsevier
external identifiers
  • wos:000246382500031
  • scopus:33947584425
ISSN
1873-4235
DOI
10.1016/j.bios.2006.10.023
language
English
LU publication?
yes
id
f635f056-535c-4538-87cd-f9b939bab7c7 (old id 660590)
date added to LUP
2007-12-06 10:16:20
date last changed
2017-04-30 14:09:14
@article{f635f056-535c-4538-87cd-f9b939bab7c7,
  abstract     = {This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Antiochia, Riccarda and Gorton, Lo},
  issn         = {1873-4235},
  keyword      = {osmium redox,diaphorase,glucose dehydrogenase,biosensor,glucose,polymer,carbon nanotube paste electrode},
  language     = {eng},
  number       = {11},
  pages        = {2611--2617},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages},
  url          = {http://dx.doi.org/10.1016/j.bios.2006.10.023},
  volume       = {22},
  year         = {2007},
}