Pituitary adenylate cyclase activating peptide: a neuropeptide in the sensory nervous system.
(1996)- Abstract
- This thesis deals with the expression of pituitary adenylate cyclase activating peptide (PACAP) in sensory C-type cells and primary afferent fibres and its possible role in nociceptive transmission. PACAP-expression, -release, -synthesis and -function were investigated in a series of measurements using immunocytochemistry, radioimmunoassay, in situ hybridization histochemistry, Northern blot, electrophysiology, superficial spinal perfusion and nerve injury. PACAP expresses normally in C-type sensory neurons and primary afferent fibres in rats and coexists with SP/CGRP in nerve fibres of the superficial laminae of the dorsal horn and of the peripheral tissues, of organs with prominent sensory innervation. Capsaicin releases PACAP from the... (More)
- This thesis deals with the expression of pituitary adenylate cyclase activating peptide (PACAP) in sensory C-type cells and primary afferent fibres and its possible role in nociceptive transmission. PACAP-expression, -release, -synthesis and -function were investigated in a series of measurements using immunocytochemistry, radioimmunoassay, in situ hybridization histochemistry, Northern blot, electrophysiology, superficial spinal perfusion and nerve injury. PACAP expresses normally in C-type sensory neurons and primary afferent fibres in rats and coexists with SP/CGRP in nerve fibres of the superficial laminae of the dorsal horn and of the peripheral tissues, of organs with prominent sensory innervation. Capsaicin releases PACAP from the central terminals of primary C-type afferents in the spinal cord, supporting the view that PACAP participates in transmission in the spinal dorsal horn as a messenger. Intrathecal administration of PACAP suppresses the C-fibre evoked flexion reflex and formalin-induced pain-related behaviour. Taken together, the evidence shows that PACAP may act as a modulator in nociception and has antinociceptive effects. Following sciatic nerve injury, the synthesis of PACAP in the dorsal root ganglia is upregulated and the concentrations of PACAP in the spinal cord and the proximal nerve stumps enhanced, indicating that PACAP plays a role in the postaxotomy repair and restoration process. It may act as a neurotropic factor or as a survival factor in adaptation to nerve injury. The findings are consistent with the hypothesis that PACAP may be important both in sensory transmission and in regeneration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/28628
- author
- Zhang, Yanzhen
- supervisor
- opponent
-
- Professor Hedner, Thomas, Sahlgrenska Sjukhuset, Göteborg
- publishing date
- 1996
- type
- Thesis
- publication status
- published
- subject
- keywords
- in situ hybridization, immunocytochemistry, peptide expression, inflammation, nerve injury, axotomy, capsaicin, pain, nociceptive, formalin test, C-fibres, sensory neurons, spinal cord, dorsal root ganglia, mRNA, neuropeptides, VIP, SP, PACAP, CGRP, Northern blot, rat, Neurology, neuropsychology, neurophysiology, Neurologi, neuropsykologi, neurofysiologi
- pages
- 168 pages
- defense location
- Segerfalk Lecture Hall, Wallenberg Neuroscience Center, Sölvegatan 17, Lund. (13.15)
- defense date
- 1999-09-19 10:15:00
- external identifiers
-
- other:ISRN: LUMEDW/MEFN--03--SE
- ISBN
- 91-628-2160-1
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008)
- id
- 661e80d1-a545-4e58-a8e3-f75bb2431070 (old id 28628)
- date added to LUP
- 2016-04-04 13:28:01
- date last changed
- 2018-11-21 21:14:11
@phdthesis{661e80d1-a545-4e58-a8e3-f75bb2431070,
abstract = {{This thesis deals with the expression of pituitary adenylate cyclase activating peptide (PACAP) in sensory C-type cells and primary afferent fibres and its possible role in nociceptive transmission. PACAP-expression, -release, -synthesis and -function were investigated in a series of measurements using immunocytochemistry, radioimmunoassay, in situ hybridization histochemistry, Northern blot, electrophysiology, superficial spinal perfusion and nerve injury. PACAP expresses normally in C-type sensory neurons and primary afferent fibres in rats and coexists with SP/CGRP in nerve fibres of the superficial laminae of the dorsal horn and of the peripheral tissues, of organs with prominent sensory innervation. Capsaicin releases PACAP from the central terminals of primary C-type afferents in the spinal cord, supporting the view that PACAP participates in transmission in the spinal dorsal horn as a messenger. Intrathecal administration of PACAP suppresses the C-fibre evoked flexion reflex and formalin-induced pain-related behaviour. Taken together, the evidence shows that PACAP may act as a modulator in nociception and has antinociceptive effects. Following sciatic nerve injury, the synthesis of PACAP in the dorsal root ganglia is upregulated and the concentrations of PACAP in the spinal cord and the proximal nerve stumps enhanced, indicating that PACAP plays a role in the postaxotomy repair and restoration process. It may act as a neurotropic factor or as a survival factor in adaptation to nerve injury. The findings are consistent with the hypothesis that PACAP may be important both in sensory transmission and in regeneration.}},
author = {{Zhang, Yanzhen}},
isbn = {{91-628-2160-1}},
keywords = {{in situ hybridization; immunocytochemistry; peptide expression; inflammation; nerve injury; axotomy; capsaicin; pain; nociceptive; formalin test; C-fibres; sensory neurons; spinal cord; dorsal root ganglia; mRNA; neuropeptides; VIP; SP; PACAP; CGRP; Northern blot; rat; Neurology; neuropsychology; neurophysiology; Neurologi; neuropsykologi; neurofysiologi}},
language = {{eng}},
title = {{Pituitary adenylate cyclase activating peptide: a neuropeptide in the sensory nervous system.}},
year = {{1996}},
}