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Characteristics of gastrin controlled ECL cell specific gene expression

Friis-Hansen, Lennart; Schjerling, Charlotte Karlskov; de la Cour, Charlotta LU ; Håkanson, Rolf LU and Rehfeld, Jens F. (2007) In Regulatory Peptides 140(3). p.153-161
Abstract
Background: The ECL cells are histamine-producing endocrine cells in the oxymic mucosa that synthesize and secrete proteins and peptides. They are the primary target for gastrin and mediate the control of gastrin on acid secretion and oxyntic mucosal growth. Knowledge of the molecular biology of the ECL cell is therefore important for understanding gastric physiology. Accordingly, we wanted to identify genes that are characteristically expressed in the ECL cells and controlled by gastrin. Methods: Using Affymetrix GeneChips((R)), RNA expression profiles were generated from ECL cells isolated by counterflow elutriation from hyper- or hypogastrinemic rats. Contamination from non-endocrine cells was eliminated by subtraction of the expression... (More)
Background: The ECL cells are histamine-producing endocrine cells in the oxymic mucosa that synthesize and secrete proteins and peptides. They are the primary target for gastrin and mediate the control of gastrin on acid secretion and oxyntic mucosal growth. Knowledge of the molecular biology of the ECL cell is therefore important for understanding gastric physiology. Accordingly, we wanted to identify genes that are characteristically expressed in the ECL cells and controlled by gastrin. Methods: Using Affymetrix GeneChips((R)), RNA expression profiles were generated from ECL cells isolated by counterflow elutriation from hyper- or hypogastrinemic rats. Contamination from non-endocrine cells was eliminated by subtraction of the expression profiles of the fundic and antral mucosa. Results: The expression of 365 genes was ECL cell characteristic. Gastrin was found to control the expression of 120 which could be divided into two major groups depending on the known or anticipated biological function of the encoded protein: genes encoding proteins involved in the secretory process and genes encoding proteins needed to generate energy for secretion. Interestingly, gastrin stimulation also increased ECL cells expression of anti-apoptotic genes. Conclusion: The ECL cell specific expression profile is reminiscent of that of neurons and other endocrine cells exhibiting high expression of genes encoding proteins involved in the synthesis, storage and secretion of neuropeptides or peptide hormones. Gastrin regulated the expression of one third of these genes and is thus involved in the control of secretion from the ECL cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gene expression, cDNA microarray, rat, ECL cells, gastrin
in
Regulatory Peptides
volume
140
issue
3
pages
153 - 161
publisher
Elsevier
external identifiers
  • wos:000246086400009
  • scopus:33947227327
ISSN
1873-1686
DOI
10.1016/j.regpep.2006.12.006
language
English
LU publication?
yes
id
39e7a8a0-7c43-4864-95ec-7e69e2f10374 (old id 662918)
date added to LUP
2007-12-07 11:16:25
date last changed
2017-01-01 04:56:03
@article{39e7a8a0-7c43-4864-95ec-7e69e2f10374,
  abstract     = {Background: The ECL cells are histamine-producing endocrine cells in the oxymic mucosa that synthesize and secrete proteins and peptides. They are the primary target for gastrin and mediate the control of gastrin on acid secretion and oxyntic mucosal growth. Knowledge of the molecular biology of the ECL cell is therefore important for understanding gastric physiology. Accordingly, we wanted to identify genes that are characteristically expressed in the ECL cells and controlled by gastrin. Methods: Using Affymetrix GeneChips((R)), RNA expression profiles were generated from ECL cells isolated by counterflow elutriation from hyper- or hypogastrinemic rats. Contamination from non-endocrine cells was eliminated by subtraction of the expression profiles of the fundic and antral mucosa. Results: The expression of 365 genes was ECL cell characteristic. Gastrin was found to control the expression of 120 which could be divided into two major groups depending on the known or anticipated biological function of the encoded protein: genes encoding proteins involved in the secretory process and genes encoding proteins needed to generate energy for secretion. Interestingly, gastrin stimulation also increased ECL cells expression of anti-apoptotic genes. Conclusion: The ECL cell specific expression profile is reminiscent of that of neurons and other endocrine cells exhibiting high expression of genes encoding proteins involved in the synthesis, storage and secretion of neuropeptides or peptide hormones. Gastrin regulated the expression of one third of these genes and is thus involved in the control of secretion from the ECL cells.},
  author       = {Friis-Hansen, Lennart and Schjerling, Charlotte Karlskov and de la Cour, Charlotta and Håkanson, Rolf and Rehfeld, Jens F.},
  issn         = {1873-1686},
  keyword      = {gene expression,cDNA microarray,rat,ECL cells,gastrin},
  language     = {eng},
  number       = {3},
  pages        = {153--161},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Characteristics of gastrin controlled ECL cell specific gene expression},
  url          = {http://dx.doi.org/10.1016/j.regpep.2006.12.006},
  volume       = {140},
  year         = {2007},
}