Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two- substrate reactions
(2007) In Analyst 132(5). p.397-402- Abstract
- The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the... (More)
- The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/666611
- author
- Shipovskov, Stepan LU and Reimann, Curt LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analyst
- volume
- 132
- issue
- 5
- pages
- 397 - 402
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000246070800003
- scopus:34247614107
- ISSN
- 1364-5528
- DOI
- 10.1039/b615394c
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
- id
- 97f8a5e4-89e8-48f5-a024-760820ca240b (old id 666611)
- date added to LUP
- 2016-04-01 15:36:13
- date last changed
- 2022-01-28 06:11:02
@article{97f8a5e4-89e8-48f5-a024-760820ca240b,
abstract = {{The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems.}},
author = {{Shipovskov, Stepan and Reimann, Curt}},
issn = {{1364-5528}},
language = {{eng}},
number = {{5}},
pages = {{397--402}},
publisher = {{Royal Society of Chemistry}},
series = {{Analyst}},
title = {{Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two- substrate reactions}},
url = {{http://dx.doi.org/10.1039/b615394c}},
doi = {{10.1039/b615394c}},
volume = {{132}},
year = {{2007}},
}