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Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two- substrate reactions

Shipovskov, Stepan LU and Reimann, Curt LU (2007) In Analyst 132(5). p.397-402
Abstract
The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the... (More)
The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
132
issue
5
pages
397 - 402
publisher
Royal Society of Chemistry
external identifiers
  • wos:000246070800003
  • scopus:34247614107
ISSN
1364-5528
DOI
10.1039/b615394c
language
English
LU publication?
yes
id
97f8a5e4-89e8-48f5-a024-760820ca240b (old id 666611)
date added to LUP
2007-12-19 14:26:37
date last changed
2017-01-01 06:42:57
@article{97f8a5e4-89e8-48f5-a024-760820ca240b,
  abstract     = {The purpose of this review is to draw attention to the use of electrospray ionization mass spectrometry ( ESI-MS) for monitoring the course of enzyme substrate interactions, in the particular case of complex systems in which two substrates participate. The determination and characterization of intra-molecular reactions, especially those that occur in the enzyme active site, is not a trivial task in chemical kinetics, typically requiring long measurement times and relatively expensive techniques such as nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy ( EM). However, nowadays almost all laboratories are equipped with or else have access to the ESI-MS technique. The aim of this review is to focus on the possibilities of employing even quite simple MS equipment to tackle different applications in studies of complex enzymatic systems.},
  author       = {Shipovskov, Stepan and Reimann, Curt},
  issn         = {1364-5528},
  language     = {eng},
  number       = {5},
  pages        = {397--402},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two- substrate reactions},
  url          = {http://dx.doi.org/10.1039/b615394c},
  volume       = {132},
  year         = {2007},
}