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The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - A yeast of biotechnological interest

Kunze, Gotthard ; Gaillardin, Claude ; Czernicka, Małgorzata ; Durrens, Pascal ; Martin, Tiphaine ; Böer, Erik ; Gabaldón, Toni ; Cruz, Jose A. ; Talla, Emmanuel and Marck, Christian , et al. (2014) In Biotechnology for Biofuels 7(1).
Abstract

Background: The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant. Results: The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific... (More)

Background: The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant. Results: The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific families appear to result from multiple rounds of segmental duplications of tandem gene arrays, a novel mechanism not yet described in yeasts. An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. Conclusions: The high-quality genome of this species that diverged early in Saccharomycotina will allow further fundamental studies on comparative genomics, evolution and phylogenetics. Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. In the course of identifying alternative microorganisms for biotechnological interest, A. adeninivorans has already proved its strengthened competitiveness as a promising cell factory for many more applications.

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keywords
Biotechnology, Genome, Metabolism, n-butanol, Tannic acid, Yeast
in
Biotechnology for Biofuels
volume
7
issue
1
article number
66
publisher
BioMed Central
external identifiers
  • scopus:84900514784
ISSN
1754-6834
DOI
10.1186/1754-6834-7-66
language
English
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no
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667057f2-41cb-4e9f-9df8-03e97ffd4d06
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2018-10-09 09:53:02
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2020-01-22 07:15:26
@article{667057f2-41cb-4e9f-9df8-03e97ffd4d06,
  abstract     = {<p>Background: The industrially important yeast Blastobotrys (Arxula) adeninivorans is an asexual hemiascomycete phylogenetically very distant from Saccharomyces cerevisiae. Its unusual metabolic flexibility allows it to use a wide range of carbon and nitrogen sources, while being thermotolerant, xerotolerant and osmotolerant. Results: The sequencing of strain LS3 revealed that the nuclear genome of A. adeninivorans is 11.8 Mb long and consists of four chromosomes with regional centromeres. Its closest sequenced relative is Yarrowia lipolytica, although mean conservation of orthologs is low. With 914 introns within 6116 genes, A. adeninivorans is one of the most intron-rich hemiascomycetes sequenced to date. Several large species-specific families appear to result from multiple rounds of segmental duplications of tandem gene arrays, a novel mechanism not yet described in yeasts. An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. Conclusions: The high-quality genome of this species that diverged early in Saccharomycotina will allow further fundamental studies on comparative genomics, evolution and phylogenetics. Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. In the course of identifying alternative microorganisms for biotechnological interest, A. adeninivorans has already proved its strengthened competitiveness as a promising cell factory for many more applications.</p>},
  author       = {Kunze, Gotthard and Gaillardin, Claude and Czernicka, Małgorzata and Durrens, Pascal and Martin, Tiphaine and Böer, Erik and Gabaldón, Toni and Cruz, Jose A. and Talla, Emmanuel and Marck, Christian and Goffeau, André and Barbe, Valérie and Baret, Philippe and Baronian, Keith and Beier, Sebastian and Bleykasten, Claudine and Bode, Rüdiger and Casaregola, Serge and Despons, Laurence and Fairhead, Cécile and Giersberg, Martin and Gierski, Przemysław Piotr and Hähnel, Urs and Hartmann, Anja and Jankowska, Dagmara and Jubin, Claire and Jung, Paul and Lafontaine, Ingrid and Leh-Louis, Véronique and Lemaire, Marc and Marcet-Houben, Marina and Mascher, Martin and Morel, Guillaume and Richard, Guy Franck and Riechen, Jan and Sacerdot, Christine and Sarkar, Anasua and Savel, Guilhem and Schacherer, Joseph and Sherman, David J. and Stein, Nils and Straub, Marie Laure and Thierry, Agnès and Trautwein-Schult, Anke and Vacherie, Benoit and Westhof, Eric and Worch, Sebastian and Dujon, Bernard and Souciet, Jean Luc and Wincker, Patrick},
  issn         = {1754-6834},
  language     = {eng},
  month        = {04},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Biotechnology for Biofuels},
  title        = {The complete genome of Blastobotrys (Arxula) adeninivorans LS3 - A yeast of biotechnological interest},
  url          = {http://dx.doi.org/10.1186/1754-6834-7-66},
  doi          = {10.1186/1754-6834-7-66},
  volume       = {7},
  year         = {2014},
}