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Immobilization of a recombinant Escherichia coli producing a thermostable alpha-L-rhamnosidase: Creation of a bioreactor for hydrolyses of naringin

Birgisson, Hakon LU ; Wheat, Jon Oskar; Hreggvidsson, Gudmundur O.; Kristjansson, Jakob K. and Mattiasson, Bo LU (2007) In Enzyme and Microbial Technology 40(5). p.1181-1187
Abstract
An U-L-rhamnosidase (E.C. 3.2.1.40) from a newly discovered thermophilic bacterium was expressed in Escherichia coli BL21 DE3 pRIL cells. The cells were immobilized in Ca2+-alginate beads. The temperature of 50 degrees C used in reactions, appeared to be sufficient for making the mesophilic strain porous enough for the substrate to access the cloned thermostable enzyme. Pretreatment of cells with heat or lysozyme prior to bead formation did not improve the results. The best cell concentration (w/w) for bead preparation was found to be 0.0 192 g ml(-1) and stability of beads increased if CaCl2 concentration in buffers and substrate was kept at 50 mM. In a 60 min assay, the optimal pH of the entrapped cells was found to be 7.8 and the... (More)
An U-L-rhamnosidase (E.C. 3.2.1.40) from a newly discovered thermophilic bacterium was expressed in Escherichia coli BL21 DE3 pRIL cells. The cells were immobilized in Ca2+-alginate beads. The temperature of 50 degrees C used in reactions, appeared to be sufficient for making the mesophilic strain porous enough for the substrate to access the cloned thermostable enzyme. Pretreatment of cells with heat or lysozyme prior to bead formation did not improve the results. The best cell concentration (w/w) for bead preparation was found to be 0.0 192 g ml(-1) and stability of beads increased if CaCl2 concentration in buffers and substrate was kept at 50 mM. In a 60 min assay, the optimal pH of the entrapped cells was found to be 7.8 and the optimal temperature 60 degrees C. By packing the beads in a column, a bioreactor for production Of L-rhamnose from naringin was created. Full degradation of 7.9 mM naringin could be reached by running the reactor at 1 ml min(-1) at 50 degrees C. The optimal running temperature of the reactor was found to be 50 degrees C and the reactor was fully stable over 3 days at that temperature. On the fourth day, substrate degradation capacity had decreased by 10-15%. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
recombinant alpha-L-rhamnosidase, immobilization, alginate, thermophilic, bioreactor
in
Enzyme and Microbial Technology
volume
40
issue
5
pages
1181 - 1187
publisher
Elsevier
external identifiers
  • wos:000245519000028
  • scopus:33847641484
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2006.08.026
language
English
LU publication?
yes
id
b1065735-8a5f-4908-9b5a-3f2109af6d30 (old id 667890)
date added to LUP
2007-12-10 11:55:30
date last changed
2017-03-05 03:26:50
@article{b1065735-8a5f-4908-9b5a-3f2109af6d30,
  abstract     = {An U-L-rhamnosidase (E.C. 3.2.1.40) from a newly discovered thermophilic bacterium was expressed in Escherichia coli BL21 DE3 pRIL cells. The cells were immobilized in Ca2+-alginate beads. The temperature of 50 degrees C used in reactions, appeared to be sufficient for making the mesophilic strain porous enough for the substrate to access the cloned thermostable enzyme. Pretreatment of cells with heat or lysozyme prior to bead formation did not improve the results. The best cell concentration (w/w) for bead preparation was found to be 0.0 192 g ml(-1) and stability of beads increased if CaCl2 concentration in buffers and substrate was kept at 50 mM. In a 60 min assay, the optimal pH of the entrapped cells was found to be 7.8 and the optimal temperature 60 degrees C. By packing the beads in a column, a bioreactor for production Of L-rhamnose from naringin was created. Full degradation of 7.9 mM naringin could be reached by running the reactor at 1 ml min(-1) at 50 degrees C. The optimal running temperature of the reactor was found to be 50 degrees C and the reactor was fully stable over 3 days at that temperature. On the fourth day, substrate degradation capacity had decreased by 10-15%. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Birgisson, Hakon and Wheat, Jon Oskar and Hreggvidsson, Gudmundur O. and Kristjansson, Jakob K. and Mattiasson, Bo},
  issn         = {0141-0229},
  keyword      = {recombinant alpha-L-rhamnosidase,immobilization,alginate,thermophilic,bioreactor},
  language     = {eng},
  number       = {5},
  pages        = {1181--1187},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Immobilization of a recombinant Escherichia coli producing a thermostable alpha-L-rhamnosidase: Creation of a bioreactor for hydrolyses of naringin},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2006.08.026},
  volume       = {40},
  year         = {2007},
}