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Controlled pilot development unit-scale fed-batch cultivation of yeast on spruce hydrolysates

Rudolf, Andreas LU ; Lequeux, Gaspard and Lidén, Gunnar LU (2007) In Biotechnology Progress 23(2). p.351-358
Abstract
Yeast production on hydrolysate is a likely process solution in large-scale ethanol production from lignocellulose. The hydrolysate will be available on site, and the yeast has furthermore been shown to acquire an increased inhibitor tolerance when cultivated on hydrolysate. However, due to over-flow metabolism and inhibition, efficient yeast production on hydrolysate can only be achieved by well-controlled substrate addition. In the present work, a method was developed for controlled addition of hydrolysate to PDU (process development unit)-scale aerobic fed-batch cultivations of Saccharomyces cerevisiae TMB 3000. A feed rate control strategy, which maintains the ethanol concentration at a low constant level, was adapted to process-like... (More)
Yeast production on hydrolysate is a likely process solution in large-scale ethanol production from lignocellulose. The hydrolysate will be available on site, and the yeast has furthermore been shown to acquire an increased inhibitor tolerance when cultivated on hydrolysate. However, due to over-flow metabolism and inhibition, efficient yeast production on hydrolysate can only be achieved by well-controlled substrate addition. In the present work, a method was developed for controlled addition of hydrolysate to PDU (process development unit)-scale aerobic fed-batch cultivations of Saccharomyces cerevisiae TMB 3000. A feed rate control strategy, which maintains the ethanol concentration at a low constant level, was adapted to process-like conditions. The ethanol concentration was obtained from on-line measurements of the ethanol mole fraction in the exhaust gas. A computer model of the system was developed to optimize control performance. Productivities, biomass yields, and byproduct formation were evaluated. The feed rate control worked satisfactorily and maintained the ethanol concentration close to the setpoint during the cultivations. Biomass yields of 0.45 g/g were obtained on added hexoses during cultivation on hydrolysate and of 0.49 g/g during cultivation on a synthetic medium with glucose as the carbon source. Exponential growth was achieved with a specific growth rate of 0.18 h(-1) during cultivation on hydrolysate and 0.22 h(-1) during cultivation on glucose. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
23
issue
2
pages
351 - 358
publisher
The American Chemical Society
external identifiers
  • wos:000245510000008
  • scopus:34247268280
ISSN
1520-6033
DOI
10.1021/bp060256u
language
English
LU publication?
yes
id
772d1fa8-68f5-42e2-9ed3-09be5ff0043b (old id 668014)
date added to LUP
2007-12-21 13:15:29
date last changed
2017-01-01 07:10:26
@article{772d1fa8-68f5-42e2-9ed3-09be5ff0043b,
  abstract     = {Yeast production on hydrolysate is a likely process solution in large-scale ethanol production from lignocellulose. The hydrolysate will be available on site, and the yeast has furthermore been shown to acquire an increased inhibitor tolerance when cultivated on hydrolysate. However, due to over-flow metabolism and inhibition, efficient yeast production on hydrolysate can only be achieved by well-controlled substrate addition. In the present work, a method was developed for controlled addition of hydrolysate to PDU (process development unit)-scale aerobic fed-batch cultivations of Saccharomyces cerevisiae TMB 3000. A feed rate control strategy, which maintains the ethanol concentration at a low constant level, was adapted to process-like conditions. The ethanol concentration was obtained from on-line measurements of the ethanol mole fraction in the exhaust gas. A computer model of the system was developed to optimize control performance. Productivities, biomass yields, and byproduct formation were evaluated. The feed rate control worked satisfactorily and maintained the ethanol concentration close to the setpoint during the cultivations. Biomass yields of 0.45 g/g were obtained on added hexoses during cultivation on hydrolysate and of 0.49 g/g during cultivation on a synthetic medium with glucose as the carbon source. Exponential growth was achieved with a specific growth rate of 0.18 h(-1) during cultivation on hydrolysate and 0.22 h(-1) during cultivation on glucose.},
  author       = {Rudolf, Andreas and Lequeux, Gaspard and Lidén, Gunnar},
  issn         = {1520-6033},
  language     = {eng},
  number       = {2},
  pages        = {351--358},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Controlled pilot development unit-scale fed-batch cultivation of yeast on spruce hydrolysates},
  url          = {http://dx.doi.org/10.1021/bp060256u},
  volume       = {23},
  year         = {2007},
}