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Tracheal remodeling: comparison of different composite cultures consisting of human respiratory epithelial cells and human chondrocytes

Pfenninger, Cosima LU ; Leinhase, Iris; Endres, Michaela; Rotter, Nicole; Loch, Alexander; Ringe, Jochen and Sittinger, Michael (2007) In In Vitro Cellular & Developmental Biology - Animal 43(1). p.28-36
Abstract
The reconstruction of extensive tracheal defects is still an unsolved challenge for thoracic surgery. Tissue engineering is a promising possibility to solve this problem through the generation of an autologous tracheal replacement from patients' own tissue. Therefore, this study investigated the potential of three different coculture systems, combining human respiratory epithelial cells and human chondrocytes. The coculture systems were analyzed by histological staining with alcian blue, immunohistochemical staining with the antibodies, 34betaE12 and CD44v6, and scanning electron microscopy. The first composite culture consisted of human respiratory epithelial cells seeded on human high-density chondrocyte pellets. For the second system,... (More)
The reconstruction of extensive tracheal defects is still an unsolved challenge for thoracic surgery. Tissue engineering is a promising possibility to solve this problem through the generation of an autologous tracheal replacement from patients' own tissue. Therefore, this study investigated the potential of three different coculture systems, combining human respiratory epithelial cells and human chondrocytes. The coculture systems were analyzed by histological staining with alcian blue, immunohistochemical staining with the antibodies, 34betaE12 and CD44v6, and scanning electron microscopy. The first composite culture consisted of human respiratory epithelial cells seeded on human high-density chondrocyte pellets. For the second system, we used native articular cartilage chips as base for the respiratory epithelial cells. The third system consisted of a collagen membrane, seeded with respiratory epithelial cells and human chondrocytes onto different sides of the membrane, which achieved the most promising results. In combination with an air-liquid interface system and fibroblast-conditioned medium, an extended epithelial multilayer with differentiated epithelial cells could be generated. Our results suggest that at least three factors are necessary for the development towards a tracheal replacement: (1) a basal lamina equivalent, consisting of collagen fibers for cell-cell interaction and cell polarization, (2) extracellular factors of mesenchymal fibroblasts, and (3) the presence of an air-liquid interface system for proliferation and differentiation of the epithelial cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CD44v6, tissue engineering, composite culture, trachea, reconstruction
in
In Vitro Cellular & Developmental Biology - Animal
volume
43
issue
1
pages
28 - 36
publisher
Springer
external identifiers
  • wos:000245377200007
  • scopus:34447634471
ISSN
1071-2690
DOI
10.1007/s11626-006-9000-6
language
English
LU publication?
yes
id
f084b642-263c-44ca-9cd3-8892b4cc6751 (old id 668473)
date added to LUP
2007-12-14 08:48:41
date last changed
2017-04-16 04:16:18
@article{f084b642-263c-44ca-9cd3-8892b4cc6751,
  abstract     = {The reconstruction of extensive tracheal defects is still an unsolved challenge for thoracic surgery. Tissue engineering is a promising possibility to solve this problem through the generation of an autologous tracheal replacement from patients' own tissue. Therefore, this study investigated the potential of three different coculture systems, combining human respiratory epithelial cells and human chondrocytes. The coculture systems were analyzed by histological staining with alcian blue, immunohistochemical staining with the antibodies, 34betaE12 and CD44v6, and scanning electron microscopy. The first composite culture consisted of human respiratory epithelial cells seeded on human high-density chondrocyte pellets. For the second system, we used native articular cartilage chips as base for the respiratory epithelial cells. The third system consisted of a collagen membrane, seeded with respiratory epithelial cells and human chondrocytes onto different sides of the membrane, which achieved the most promising results. In combination with an air-liquid interface system and fibroblast-conditioned medium, an extended epithelial multilayer with differentiated epithelial cells could be generated. Our results suggest that at least three factors are necessary for the development towards a tracheal replacement: (1) a basal lamina equivalent, consisting of collagen fibers for cell-cell interaction and cell polarization, (2) extracellular factors of mesenchymal fibroblasts, and (3) the presence of an air-liquid interface system for proliferation and differentiation of the epithelial cells.},
  author       = {Pfenninger, Cosima and Leinhase, Iris and Endres, Michaela and Rotter, Nicole and Loch, Alexander and Ringe, Jochen and Sittinger, Michael},
  issn         = {1071-2690},
  keyword      = {CD44v6,tissue engineering,composite culture,trachea,reconstruction},
  language     = {eng},
  number       = {1},
  pages        = {28--36},
  publisher    = {Springer},
  series       = {In Vitro Cellular & Developmental Biology - Animal},
  title        = {Tracheal remodeling: comparison of different composite cultures consisting of human respiratory epithelial cells and human chondrocytes},
  url          = {http://dx.doi.org/10.1007/s11626-006-9000-6},
  volume       = {43},
  year         = {2007},
}