Human complex-forming glycoprotein, heterogeneous in charge : the primary structure around the cysteine residues and characterization of a disulfide bridge
(1982) In Archives of Biochemistry and Biophysics 213(1). p.50-240- Abstract
- The amino acid sequence of the cysteine-containing regions of human complex-forming glycoprotein, heterogeneous in charge (protein HC) was determined by studies of the tryptic peptides of the completely reduced and radioalkylated protein. One of the cysteines was located in the amino-terminal part of the molecule at position 34....
Diagonal map electrophoresis showed that the cysteine residue in the carboxy-terminal region was bridged to the cysteine containing sequence in the middle of the molecule. The function of the cysteine residue at position 34 remains elusive since the residue was not found on the diagonal maps. The release of cysteic acid and a small cysteic acid containing peptide after oxidation of the native protein HC... (More) - The amino acid sequence of the cysteine-containing regions of human complex-forming glycoprotein, heterogeneous in charge (protein HC) was determined by studies of the tryptic peptides of the completely reduced and radioalkylated protein. One of the cysteines was located in the amino-terminal part of the molecule at position 34....
Diagonal map electrophoresis showed that the cysteine residue in the carboxy-terminal region was bridged to the cysteine containing sequence in the middle of the molecule. The function of the cysteine residue at position 34 remains elusive since the residue was not found on the diagonal maps. The release of cysteic acid and a small cysteic acid containing peptide after oxidation of the native protein HC molecule suggests that this cysteine residue may be involved in disulfide bridges with cysteine and small cysteine containing peptides. (Less)
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- author
- Mendez, E ; Grubb, A O LU ; Lopez, C ; Frangione, B and Franklin, E C
- organization
- publishing date
- 1982
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alpha-Globulins/analysis, Amino Acid Sequence, Chromatography, Gel, Cyanogen Bromide, Cysteine/analysis, Humans, Trypsin/metabolism
- in
- Archives of Biochemistry and Biophysics
- volume
- 213
- issue
- 1
- pages
- 50 - 240
- publisher
- Academic Press
- external identifiers
-
- pmid:6174078
- scopus:0020013957
- ISSN
- 0003-9861
- DOI
- 10.1016/0003-9861(82)90458-1
- language
- English
- LU publication?
- yes
- id
- 6690cd59-1c35-4d59-9c76-afc5d38b3806
- date added to LUP
- 2021-10-22 15:25:05
- date last changed
- 2024-01-12 02:57:58
@article{6690cd59-1c35-4d59-9c76-afc5d38b3806, abstract = {{The amino acid sequence of the cysteine-containing regions of human complex-forming glycoprotein, heterogeneous in charge (protein HC) was determined by studies of the tryptic peptides of the completely reduced and radioalkylated protein. One of the cysteines was located in the amino-terminal part of the molecule at position 34....<br/>Diagonal map electrophoresis showed that the cysteine residue in the carboxy-terminal region was bridged to the cysteine containing sequence in the middle of the molecule. The function of the cysteine residue at position 34 remains elusive since the residue was not found on the diagonal maps. The release of cysteic acid and a small cysteic acid containing peptide after oxidation of the native protein HC molecule suggests that this cysteine residue may be involved in disulfide bridges with cysteine and small cysteine containing peptides.}}, author = {{Mendez, E and Grubb, A O and Lopez, C and Frangione, B and Franklin, E C}}, issn = {{0003-9861}}, keywords = {{Alpha-Globulins/analysis; Amino Acid Sequence; Chromatography, Gel; Cyanogen Bromide; Cysteine/analysis; Humans; Trypsin/metabolism}}, language = {{eng}}, number = {{1}}, pages = {{50--240}}, publisher = {{Academic Press}}, series = {{Archives of Biochemistry and Biophysics}}, title = {{Human complex-forming glycoprotein, heterogeneous in charge : the primary structure around the cysteine residues and characterization of a disulfide bridge}}, url = {{http://dx.doi.org/10.1016/0003-9861(82)90458-1}}, doi = {{10.1016/0003-9861(82)90458-1}}, volume = {{213}}, year = {{1982}}, }