A tumor necrosis factor binding protein is present in human biological fluids
Peetre, Christina ; Thysell, Hans LU ; Grubb, A LU
and Olsson, Inge
LU
(1988)
In European Journal of Haematology
41(5).
p.414-419
- Abstract
Tumor necrosis factor (TNF) possesses both beneficial and toxic bioactivities. Mechanisms may operate to counteract harmful effects. We have identified a TNF binding protein (TNF-BP), which shows increased levels in serum and urine of patients on regular hemodialysis treatment (RDT). TNF-BP inhibited the specific binding of human recombinant TNF (rTNF) to its cell surface receptor. Results from gel chromatography demonstrated the presence in serum and urine of a macromolecule with an apparent molecular weight of 50,000, which formed a complex with rTNF. A 62-fold purification of TNF-BP from urine of patients on RDT was achieved by ion exchange chromatography and gel chromatography. Partially purified TNF-BP reduced the growth inhibitory... (More)
Tumor necrosis factor (TNF) possesses both beneficial and toxic bioactivities. Mechanisms may operate to counteract harmful effects. We have identified a TNF binding protein (TNF-BP), which shows increased levels in serum and urine of patients on regular hemodialysis treatment (RDT). TNF-BP inhibited the specific binding of human recombinant TNF (rTNF) to its cell surface receptor. Results from gel chromatography demonstrated the presence in serum and urine of a macromolecule with an apparent molecular weight of 50,000, which formed a complex with rTNF. A 62-fold purification of TNF-BP from urine of patients on RDT was achieved by ion exchange chromatography and gel chromatography. Partially purified TNF-BP reduced the growth inhibitory effect of rTNF on a susceptible leukemia cell line. TNF-BP may act as a regulator of the biological activity of TNF and could have beneficial effects in certain inflammatory conditions.
(Less)
- author
- Peetre, Christina
; Thysell, Hans
LU
; Grubb, A
LU
and Olsson, Inge
LU
- organization
- publishing date
- 1988
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Binding, Competitive, Chromatography, Gel, Chromatography, Ion Exchange, Humans, Kidney Diseases/blood, Protein Binding, Receptors, Cell Surface/isolation & purification, Recombinant Proteins/metabolism, Renal Dialysis, Tumor Cells, Cultured/drug effects, Tumor Necrosis Factor-alpha/antagonists & inhibitors
- in
- European Journal of Haematology
- volume
- 41
- issue
- 5
- pages
- 414 - 419
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0024214443
- pmid:2850215
- ISSN
- 0902-4441
- DOI
- 10.1111/j.1600-0609.1988.tb00220.x
- language
- English
- LU publication?
- yes
- id
- 6691d36e-c7c6-481a-9b24-68cdb5efe461
- date added to LUP
- 2021-10-27 08:23:53
- date last changed
- 2024-01-12 02:54:29
@article{6691d36e-c7c6-481a-9b24-68cdb5efe461,
abstract = {{<p>Tumor necrosis factor (TNF) possesses both beneficial and toxic bioactivities. Mechanisms may operate to counteract harmful effects. We have identified a TNF binding protein (TNF-BP), which shows increased levels in serum and urine of patients on regular hemodialysis treatment (RDT). TNF-BP inhibited the specific binding of human recombinant TNF (rTNF) to its cell surface receptor. Results from gel chromatography demonstrated the presence in serum and urine of a macromolecule with an apparent molecular weight of 50,000, which formed a complex with rTNF. A 62-fold purification of TNF-BP from urine of patients on RDT was achieved by ion exchange chromatography and gel chromatography. Partially purified TNF-BP reduced the growth inhibitory effect of rTNF on a susceptible leukemia cell line. TNF-BP may act as a regulator of the biological activity of TNF and could have beneficial effects in certain inflammatory conditions.</p>}},
author = {{Peetre, Christina and Thysell, Hans and Grubb, A and Olsson, Inge}},
issn = {{0902-4441}},
keywords = {{Binding, Competitive; Chromatography, Gel; Chromatography, Ion Exchange; Humans; Kidney Diseases/blood; Protein Binding; Receptors, Cell Surface/isolation & purification; Recombinant Proteins/metabolism; Renal Dialysis; Tumor Cells, Cultured/drug effects; Tumor Necrosis Factor-alpha/antagonists & inhibitors}},
language = {{eng}},
number = {{5}},
pages = {{414--419}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Haematology}},
title = {{A tumor necrosis factor binding protein is present in human biological fluids}},
url = {{http://dx.doi.org/10.1111/j.1600-0609.1988.tb00220.x}},
doi = {{10.1111/j.1600-0609.1988.tb00220.x}},
volume = {{41}},
year = {{1988}},
}