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Nuclear microscopy: A tool for and percutaneous absorption imaging elemental distribution in vivo

Verissimo, Ana; Alves, Luis C.; Filipe, Paulo; Silva, Joao N.; Silva, Raquel; Ynsa, Maria Dolores; Gontier, Etienne; Moretto, Philippe; Pallon, Jan LU and Pinheiro, Teresa (2007) In Microscopy Research and Technique 70(4). p.302-309
Abstract
Nuclear microscopy is a technique based on a focused beam of accelerated particles that has the ability of imaging the morphology of the tissue in vivo and of producing the correspondent elemental maps, whether in major, minor, or trace concentrations. These characteristics constitute a strong advantage in studying the morphology of human skin, its elemental distributions and the permeation mechanisms of chemical compounds. In this study, nuclear microscopy techniques such as scanning transmission ion microscopy and particle induced X-ray emission were applied simultaneously, to cryopreserved human skin samples with the purpose of obtaining high-resolution images of cells and tissue morphology. In addition, quantitative elemental profiling... (More)
Nuclear microscopy is a technique based on a focused beam of accelerated particles that has the ability of imaging the morphology of the tissue in vivo and of producing the correspondent elemental maps, whether in major, minor, or trace concentrations. These characteristics constitute a strong advantage in studying the morphology of human skin, its elemental distributions and the permeation mechanisms of chemical compounds. In this study, nuclear microscopy techniques such as scanning transmission ion microscopy and particle induced X-ray emission were applied simultaneously, to cryopreserved human skin samples with the purpose of obtaining high-resolution images of cells and tissue morphology. In addition, quantitative elemental profiling and mapping of phosphorus, calcium, chlorine, and potassium in skin cross-sections were obtained. This procedure accurately distinguishes the epidermal strata and dermis by overlapping in real time the elemental information with density images obtained from the transmitted beam. A validation procedure for elemental distributions in human skin based on differential density of epidermal strata and dermis was established. As demonstrated, this procedure can be used in future studies as a tool for the in vivo examination of trans-epidermal and -dermal delivery of products. . (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
human skin, X-ray microscopy, PIXE, STIM, metals
in
Microscopy Research and Technique
volume
70
issue
4
pages
302 - 309
publisher
John Wiley & Sons
external identifiers
  • wos:000245596000003
  • scopus:34147206319
ISSN
1059-910X
DOI
10.1002/jemt.20402
language
English
LU publication?
yes
id
7bb5f6d5-8f3c-4d7a-9717-07bc3e5e78a2 (old id 669327)
date added to LUP
2008-01-03 11:24:52
date last changed
2017-09-10 03:39:16
@article{7bb5f6d5-8f3c-4d7a-9717-07bc3e5e78a2,
  abstract     = {Nuclear microscopy is a technique based on a focused beam of accelerated particles that has the ability of imaging the morphology of the tissue in vivo and of producing the correspondent elemental maps, whether in major, minor, or trace concentrations. These characteristics constitute a strong advantage in studying the morphology of human skin, its elemental distributions and the permeation mechanisms of chemical compounds. In this study, nuclear microscopy techniques such as scanning transmission ion microscopy and particle induced X-ray emission were applied simultaneously, to cryopreserved human skin samples with the purpose of obtaining high-resolution images of cells and tissue morphology. In addition, quantitative elemental profiling and mapping of phosphorus, calcium, chlorine, and potassium in skin cross-sections were obtained. This procedure accurately distinguishes the epidermal strata and dermis by overlapping in real time the elemental information with density images obtained from the transmitted beam. A validation procedure for elemental distributions in human skin based on differential density of epidermal strata and dermis was established. As demonstrated, this procedure can be used in future studies as a tool for the in vivo examination of trans-epidermal and -dermal delivery of products. .},
  author       = {Verissimo, Ana and Alves, Luis C. and Filipe, Paulo and Silva, Joao N. and Silva, Raquel and Ynsa, Maria Dolores and Gontier, Etienne and Moretto, Philippe and Pallon, Jan and Pinheiro, Teresa},
  issn         = {1059-910X},
  keyword      = {human skin,X-ray microscopy,PIXE,STIM,metals},
  language     = {eng},
  number       = {4},
  pages        = {302--309},
  publisher    = {John Wiley & Sons},
  series       = {Microscopy Research and Technique},
  title        = {Nuclear microscopy: A tool for and percutaneous absorption imaging elemental distribution in vivo},
  url          = {http://dx.doi.org/10.1002/jemt.20402},
  volume       = {70},
  year         = {2007},
}