Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands
Deraz, Sahar LU ; Plieva, Fatima LU ; Galaev, Igor LU ; Nordberg Karlsson, Eva LU
and Mattiasson, Bo
LU
(2007)
In Enzyme and Microbial Technology
40(4).
p.786-793
- Abstract
- The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a... (More)
- The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/669835
- author
- Deraz, Sahar
LU
; Plieva, Fatima
LU
; Galaev, Igor
LU
; Nordberg Karlsson, Eva
LU
and Mattiasson, Bo
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cryogel in microtiter plate, macroporous monolith, bacteriocin, integrated product adsorption
- in
- Enzyme and Microbial Technology
- volume
- 40
- issue
- 4
- pages
- 786 - 793
- publisher
- Elsevier
- external identifiers
-
- wos:000245015600040
- scopus:33847309501
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2006.06.024
- language
- English
- LU publication?
- yes
- id
- 02431b5c-d0b0-498e-a5e1-faf92cca7f45 (old id 669835)
- date added to LUP
- 2016-04-01 11:52:26
- date last changed
- 2022-01-26 19:31:37
@article{02431b5c-d0b0-498e-a5e1-faf92cca7f45,
abstract = {{The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples.}},
author = {{Deraz, Sahar and Plieva, Fatima and Galaev, Igor and Nordberg Karlsson, Eva and Mattiasson, Bo}},
issn = {{0141-0229}},
keywords = {{cryogel in microtiter plate; macroporous monolith; bacteriocin; integrated product adsorption}},
language = {{eng}},
number = {{4}},
pages = {{786--793}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2006.06.024}},
doi = {{10.1016/j.enzmictec.2006.06.024}},
volume = {{40}},
year = {{2007}},
}