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Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands

Deraz, Sahar LU ; Plieva, Fatima LU ; Galaev, Igor LU ; Nordberg Karlsson, Eva LU and Mattiasson, Bo LU (2007) In Enzyme and Microbial Technology 40(4). p.786-793
Abstract
The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a... (More)
The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cryogel in microtiter plate, macroporous monolith, bacteriocin, integrated product adsorption
in
Enzyme and Microbial Technology
volume
40
issue
4
pages
786 - 793
publisher
Elsevier
external identifiers
  • wos:000245015600040
  • scopus:33847309501
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2006.06.024
language
English
LU publication?
yes
id
02431b5c-d0b0-498e-a5e1-faf92cca7f45 (old id 669835)
date added to LUP
2007-12-10 14:30:49
date last changed
2017-02-05 03:32:18
@article{02431b5c-d0b0-498e-a5e1-faf92cca7f45,
  abstract     = {The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples.},
  author       = {Deraz, Sahar and Plieva, Fatima and Galaev, Igor and Nordberg Karlsson, Eva and Mattiasson, Bo},
  issn         = {0141-0229},
  keyword      = {cryogel in microtiter plate,macroporous monolith,bacteriocin,integrated product adsorption},
  language     = {eng},
  number       = {4},
  pages        = {786--793},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2006.06.024},
  volume       = {40},
  year         = {2007},
}