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Rapid high throughput assay for fluorimetric detection of doxorubicin - Application of nucleic acid-dye bioprobe

Liu, Yang LU and Danielsson, Bengt LU (2007) In Analytica Chimica Acta 587(1). p.47-51
Abstract
A double stranded DNA based fluorescence bioprobe for anticancer agent (doxorubicin) detection is described. This method provides a new way for sensitive DNA/drug interaction study by a homogeneous assay. The probe employs the long-wavelength intercalating fluorophore TOTO-3((R)) (TT3). The anticancer agent, doxorubicin, which interacts with the DNA-TT3 complex, was indirectly measured by the decrease in the fluorescence intensity. Various oligonucleotides with different sequences were examined. Doxorubicin has preference for the oligonucleotide 5'AGCACG3'. Enhanced fluorescence observed for the TT3 intercalation with this oligonucleotide makes the DNA-dye complex a suitable bioprobe for doxorubicin detection by competitive assay. A... (More)
A double stranded DNA based fluorescence bioprobe for anticancer agent (doxorubicin) detection is described. This method provides a new way for sensitive DNA/drug interaction study by a homogeneous assay. The probe employs the long-wavelength intercalating fluorophore TOTO-3((R)) (TT3). The anticancer agent, doxorubicin, which interacts with the DNA-TT3 complex, was indirectly measured by the decrease in the fluorescence intensity. Various oligonucleotides with different sequences were examined. Doxorubicin has preference for the oligonucleotide 5'AGCACG3'. Enhanced fluorescence observed for the TT3 intercalation with this oligonucleotide makes the DNA-dye complex a suitable bioprobe for doxorubicin detection by competitive assay. A home-built CCD camera setup was applied along with 384 well plate assay format for high throughput fluorescence imaging. The detection limit can be as low as 25 ng mL(-1) with an upper limit of 100 mu g mL(-1). The recovery test with spiked serum sample shows that this method can be a potential routine method for therapeutic drug monitoring (TDM). (c) 2007 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
deoxyribonucleic acid, doxorubicin, fluorescence, high throughput
in
Analytica Chimica Acta
volume
587
issue
1
pages
47 - 51
publisher
Elsevier
external identifiers
  • wos:000245158700007
  • scopus:33847252119
ISSN
1873-4324
DOI
10.1016/j.aca.2007.01.013
language
English
LU publication?
yes
id
af883076-b0cb-463a-b375-f141defc6c40 (old id 669967)
date added to LUP
2007-12-13 09:06:21
date last changed
2017-06-11 04:32:53
@article{af883076-b0cb-463a-b375-f141defc6c40,
  abstract     = {A double stranded DNA based fluorescence bioprobe for anticancer agent (doxorubicin) detection is described. This method provides a new way for sensitive DNA/drug interaction study by a homogeneous assay. The probe employs the long-wavelength intercalating fluorophore TOTO-3((R)) (TT3). The anticancer agent, doxorubicin, which interacts with the DNA-TT3 complex, was indirectly measured by the decrease in the fluorescence intensity. Various oligonucleotides with different sequences were examined. Doxorubicin has preference for the oligonucleotide 5'AGCACG3'. Enhanced fluorescence observed for the TT3 intercalation with this oligonucleotide makes the DNA-dye complex a suitable bioprobe for doxorubicin detection by competitive assay. A home-built CCD camera setup was applied along with 384 well plate assay format for high throughput fluorescence imaging. The detection limit can be as low as 25 ng mL(-1) with an upper limit of 100 mu g mL(-1). The recovery test with spiked serum sample shows that this method can be a potential routine method for therapeutic drug monitoring (TDM). (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Liu, Yang and Danielsson, Bengt},
  issn         = {1873-4324},
  keyword      = {deoxyribonucleic acid,doxorubicin,fluorescence,high throughput},
  language     = {eng},
  number       = {1},
  pages        = {47--51},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Rapid high throughput assay for fluorimetric detection of doxorubicin - Application of nucleic acid-dye bioprobe},
  url          = {http://dx.doi.org/10.1016/j.aca.2007.01.013},
  volume       = {587},
  year         = {2007},
}