Optimizing conditions for poly(beta-hydroxybutyrate) production by Halomonas boliviensis LC1 in batch culture with sucrose as carbon source
(2007) In Applied Microbiology and Biotechnology 74(5). p.981-986- Abstract
- Halomonas boliviensis LC1 is able to accumulate poly(beta-hydroxybutyrate) (PHB) under conditions of excess carbon source and depletion of essential nutrients. This study was aimed at an efficient production of PHB by growing H. boliviensis to high cell concentrations in batch cultures. The effect of ammonium, phosphate, and yeast extract concentrations on cell concentration [cell dry weight (CDW)] and PHB content of H. boliviensis cultured in shake flasks was assayed using a factorial design. High concentrations of these nutrients led to increments in cell growth but reduced the PHB content to some extent. Cultivations of H. boliviensis under controlled conditions in a fermentor using 1.5% (w/v) yeast extract as N source, and intermittent... (More)
- Halomonas boliviensis LC1 is able to accumulate poly(beta-hydroxybutyrate) (PHB) under conditions of excess carbon source and depletion of essential nutrients. This study was aimed at an efficient production of PHB by growing H. boliviensis to high cell concentrations in batch cultures. The effect of ammonium, phosphate, and yeast extract concentrations on cell concentration [cell dry weight (CDW)] and PHB content of H. boliviensis cultured in shake flasks was assayed using a factorial design. High concentrations of these nutrients led to increments in cell growth but reduced the PHB content to some extent. Cultivations of H. boliviensis under controlled conditions in a fermentor using 1.5% (w/v) yeast extract as N source, and intermittent addition of sucrose to provide excess C source, resulted in a polymer accumulation of 44 wt.% and 12 g l(-1) CDW after 24 h of cultivation. Batch cultures in a fermentor with initial concentrations of 2.5% (w/v) sucrose and 1.5% (w/v) yeast extract, and with induced oxygen limitation, resulted in an optimum PHB accumulation, PHB concentration and CDW of 54 wt.%, 7.7 g l(-1) and 14 g l(-1), respectively, after 19 h of cultivation. The addition of casaminoacids in the medium increased the CDW to 14.4 g l(-1) in 17 h but reduced the PHB content in the cells to 52 wt.%. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/670289
- author
- Quillaguaman, Jorge LU ; Munoz, Marlene LU ; Mattiasson, Bo LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (PHB) production, poly(beta-hydroxybutyrate), Halomonas boliviensis, moderate halophile
- in
- Applied Microbiology and Biotechnology
- volume
- 74
- issue
- 5
- pages
- 981 - 986
- publisher
- Springer
- external identifiers
-
- wos:000244996300005
- scopus:33947259790
- ISSN
- 1432-0614
- DOI
- 10.1007/s00253-006-0754-2
- language
- English
- LU publication?
- yes
- id
- 8ba0cdf5-9f79-458f-b8ff-29b94bf79762 (old id 670289)
- date added to LUP
- 2016-04-01 16:13:29
- date last changed
- 2022-04-07 03:54:08
@article{8ba0cdf5-9f79-458f-b8ff-29b94bf79762,
abstract = {{Halomonas boliviensis LC1 is able to accumulate poly(beta-hydroxybutyrate) (PHB) under conditions of excess carbon source and depletion of essential nutrients. This study was aimed at an efficient production of PHB by growing H. boliviensis to high cell concentrations in batch cultures. The effect of ammonium, phosphate, and yeast extract concentrations on cell concentration [cell dry weight (CDW)] and PHB content of H. boliviensis cultured in shake flasks was assayed using a factorial design. High concentrations of these nutrients led to increments in cell growth but reduced the PHB content to some extent. Cultivations of H. boliviensis under controlled conditions in a fermentor using 1.5% (w/v) yeast extract as N source, and intermittent addition of sucrose to provide excess C source, resulted in a polymer accumulation of 44 wt.% and 12 g l(-1) CDW after 24 h of cultivation. Batch cultures in a fermentor with initial concentrations of 2.5% (w/v) sucrose and 1.5% (w/v) yeast extract, and with induced oxygen limitation, resulted in an optimum PHB accumulation, PHB concentration and CDW of 54 wt.%, 7.7 g l(-1) and 14 g l(-1), respectively, after 19 h of cultivation. The addition of casaminoacids in the medium increased the CDW to 14.4 g l(-1) in 17 h but reduced the PHB content in the cells to 52 wt.%.}},
author = {{Quillaguaman, Jorge and Munoz, Marlene and Mattiasson, Bo and Hatti-Kaul, Rajni}},
issn = {{1432-0614}},
keywords = {{(PHB) production; poly(beta-hydroxybutyrate); Halomonas boliviensis; moderate halophile}},
language = {{eng}},
number = {{5}},
pages = {{981--986}},
publisher = {{Springer}},
series = {{Applied Microbiology and Biotechnology}},
title = {{Optimizing conditions for poly(beta-hydroxybutyrate) production by Halomonas boliviensis LC1 in batch culture with sucrose as carbon source}},
url = {{http://dx.doi.org/10.1007/s00253-006-0754-2}},
doi = {{10.1007/s00253-006-0754-2}},
volume = {{74}},
year = {{2007}},
}