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Prevotella bivia can invade human cervix epithelial (HeLa) cells

Strombeck, Louise; Sandors, Jens; Holst, Elisabet LU ; Madianos, Phoebus; Nannmark, Ulf; Papapanou, Panos and Mattsby-Baltzer, Inger (2007) In Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS) 115(3). p.241-251
Abstract
Prevotella bivia has been associated with female upper genital tract infections and an increased risk of preterm delivery. In this study, the adherence and invasion capacity of P. bivia was investigated using a cervix epithelial cell line. P. bivia was furthermore analysed for its ability to evoke a proinflammatory cytokine response in epithelial cells. The invasion capacity, defined as the number of bacteria recovered from lysed HeLa cells infected with P. bivia, varied considerably among five strains, all of which were isolates from women with bacterial vaginosis. One P. bivia strain (P47) gave rise to an approximately 120-fold higher number of intracellular bacteria (7 X 10(3) bacteria per 1 X 10(5) cells) compared with the least... (More)
Prevotella bivia has been associated with female upper genital tract infections and an increased risk of preterm delivery. In this study, the adherence and invasion capacity of P. bivia was investigated using a cervix epithelial cell line. P. bivia was furthermore analysed for its ability to evoke a proinflammatory cytokine response in epithelial cells. The invasion capacity, defined as the number of bacteria recovered from lysed HeLa cells infected with P. bivia, varied considerably among five strains, all of which were isolates from women with bacterial vaginosis. One P. bivia strain (P47) gave rise to an approximately 120-fold higher number of intracellular bacteria (7 X 10(3) bacteria per 1 X 10(5) cells) compared with the least invasive strain. Three strains expressed an intermediate or low invasiveness, showing an approximately 3- to 40-fold higher number of intracellular bacteria per 1 X 10(5) cells compared with the least invasive strain. The intracellular localization of P47 in phagosome-like vesicles was confirmed by transmission electron microscopy. All P. bivia strains adhered to HeLa cells to the same extent (range 14-22 bacteria per cell) as analysed by interference microscopy. No correlation was found between adhesion and invasion capacity of the strains. Furthermore, no fimbriae-like structures were observed on P47 detected by scanning electron microscopy or negative staining. Analysis of TNF-alpha, IL-1 alpha, IL-6, IL-8, and IL-18 in P. bivia-stimulated HeLa cells showed low levels of only IL-6 and IL-8 for the most invasive P. bivia strain P47. Thus, the induction of IL-6 or IL-8 secretion appeared to be associated with invasion capacity. This work provides evidence that some P. bivia isolates can invade human cervix epithelial. Thus, a strong capacity for invasion and a weak proinflammatory cytokine-inducing capacity in P. bivia are suggested to be virulence factors in establishing a low-grade upper genital tract infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adhesion, Prevotella bivia, intracellular invasion, IL-6, HeLa
in
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
volume
115
issue
3
pages
241 - 251
publisher
John Wiley & Sons
external identifiers
  • wos:000244881000009
  • scopus:33947303676
ISSN
1600-0463
DOI
10.1111/j.1600-0463.2007.apm_512.x
language
English
LU publication?
yes
id
7e512c4d-0af7-4b9c-ad12-5a96420d566a (old id 670812)
date added to LUP
2008-01-03 08:59:15
date last changed
2017-07-30 03:33:49
@article{7e512c4d-0af7-4b9c-ad12-5a96420d566a,
  abstract     = {Prevotella bivia has been associated with female upper genital tract infections and an increased risk of preterm delivery. In this study, the adherence and invasion capacity of P. bivia was investigated using a cervix epithelial cell line. P. bivia was furthermore analysed for its ability to evoke a proinflammatory cytokine response in epithelial cells. The invasion capacity, defined as the number of bacteria recovered from lysed HeLa cells infected with P. bivia, varied considerably among five strains, all of which were isolates from women with bacterial vaginosis. One P. bivia strain (P47) gave rise to an approximately 120-fold higher number of intracellular bacteria (7 X 10(3) bacteria per 1 X 10(5) cells) compared with the least invasive strain. Three strains expressed an intermediate or low invasiveness, showing an approximately 3- to 40-fold higher number of intracellular bacteria per 1 X 10(5) cells compared with the least invasive strain. The intracellular localization of P47 in phagosome-like vesicles was confirmed by transmission electron microscopy. All P. bivia strains adhered to HeLa cells to the same extent (range 14-22 bacteria per cell) as analysed by interference microscopy. No correlation was found between adhesion and invasion capacity of the strains. Furthermore, no fimbriae-like structures were observed on P47 detected by scanning electron microscopy or negative staining. Analysis of TNF-alpha, IL-1 alpha, IL-6, IL-8, and IL-18 in P. bivia-stimulated HeLa cells showed low levels of only IL-6 and IL-8 for the most invasive P. bivia strain P47. Thus, the induction of IL-6 or IL-8 secretion appeared to be associated with invasion capacity. This work provides evidence that some P. bivia isolates can invade human cervix epithelial. Thus, a strong capacity for invasion and a weak proinflammatory cytokine-inducing capacity in P. bivia are suggested to be virulence factors in establishing a low-grade upper genital tract infection.},
  author       = {Strombeck, Louise and Sandors, Jens and Holst, Elisabet and Madianos, Phoebus and Nannmark, Ulf and Papapanou, Panos and Mattsby-Baltzer, Inger},
  issn         = {1600-0463},
  keyword      = {adhesion,Prevotella bivia,intracellular invasion,IL-6,HeLa},
  language     = {eng},
  number       = {3},
  pages        = {241--251},
  publisher    = {John Wiley & Sons},
  series       = {Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)},
  title        = {Prevotella bivia can invade human cervix epithelial (HeLa) cells},
  url          = {http://dx.doi.org/10.1111/j.1600-0463.2007.apm_512.x},
  volume       = {115},
  year         = {2007},
}