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Determination of reducing ends with flow injection analysis with amperometric detection: application to enzyme-hydrolysed methyl cellulose

Melander, Claes LU ; Andersson, Emma; Axelsson, Sara and Gorton, Lo LU (2007) In Analytical and Bioanalytical Chemistry 387(7). p.2585-2593
Abstract
A novel method for detection of reducing ends of sugars is proposed, based on the use of Fe(CN)(6)(3-) as the oxidant in combination with amperometric detection and flow injection analysis (FIA). The method is very sensitive, giving values of < 10 mu M for the limit of detection for a series of mono- and oligosaccharides. Samples can be analysed every 30 s, and injection can be made fully automated, making it possible to perform on-line analysis of polysaccharide samples subjected to hydrolysis. Three methylcelluloses (MC) of different qualities were hydrolysed with three different glucanases, and the concentrations of reducing ends prior to, during and after hydrolysis were determined. Differences were observed between the results... (More)
A novel method for detection of reducing ends of sugars is proposed, based on the use of Fe(CN)(6)(3-) as the oxidant in combination with amperometric detection and flow injection analysis (FIA). The method is very sensitive, giving values of < 10 mu M for the limit of detection for a series of mono- and oligosaccharides. Samples can be analysed every 30 s, and injection can be made fully automated, making it possible to perform on-line analysis of polysaccharide samples subjected to hydrolysis. Three methylcelluloses (MC) of different qualities were hydrolysed with three different glucanases, and the concentrations of reducing ends prior to, during and after hydrolysis were determined. Differences were observed between the results obtained using different combinations of enzymes and MCs, which revealed different selectivities of the various enzymes for the different substrates. One MC was also hydrolysed and analysed in real-time for three hours. The method proposed is superior to many of the standard methods used today, which require manual labour and have a lower sensitivity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
methylcellulose, glucanases, hexacyanoferrate, on-line real time analysis
in
Analytical and Bioanalytical Chemistry
volume
387
issue
7
pages
2585 - 2593
publisher
Springer
external identifiers
  • wos:000244748500030
  • scopus:33847734335
ISSN
1618-2642
DOI
10.1007/s00216-006-1107-9
language
English
LU publication?
yes
id
10ccfa74-5247-4fac-860f-719538857eae (old id 670820)
date added to LUP
2007-12-15 14:49:19
date last changed
2017-01-01 05:06:08
@article{10ccfa74-5247-4fac-860f-719538857eae,
  abstract     = {A novel method for detection of reducing ends of sugars is proposed, based on the use of Fe(CN)(6)(3-) as the oxidant in combination with amperometric detection and flow injection analysis (FIA). The method is very sensitive, giving values of &lt; 10 mu M for the limit of detection for a series of mono- and oligosaccharides. Samples can be analysed every 30 s, and injection can be made fully automated, making it possible to perform on-line analysis of polysaccharide samples subjected to hydrolysis. Three methylcelluloses (MC) of different qualities were hydrolysed with three different glucanases, and the concentrations of reducing ends prior to, during and after hydrolysis were determined. Differences were observed between the results obtained using different combinations of enzymes and MCs, which revealed different selectivities of the various enzymes for the different substrates. One MC was also hydrolysed and analysed in real-time for three hours. The method proposed is superior to many of the standard methods used today, which require manual labour and have a lower sensitivity.},
  author       = {Melander, Claes and Andersson, Emma and Axelsson, Sara and Gorton, Lo},
  issn         = {1618-2642},
  keyword      = {methylcellulose,glucanases,hexacyanoferrate,on-line real time analysis},
  language     = {eng},
  number       = {7},
  pages        = {2585--2593},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Determination of reducing ends with flow injection analysis with amperometric detection: application to enzyme-hydrolysed methyl cellulose},
  url          = {http://dx.doi.org/10.1007/s00216-006-1107-9},
  volume       = {387},
  year         = {2007},
}