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Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations

Long, Manhai; Stronati, Alessandra; Bizzaro, Davide; Kruger, Tanja; Manicardi, Gian-Carlo; Hjelmborg, Philip S.; Spano, Marcello; Giwercman, Aleksander LU ; Toft, Gunnar and Bonde, Jens Peter, et al. (2007) In Reproduction 133(2). p.517-530
Abstract
Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay... (More)
Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population. (Less)
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publication status
published
subject
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Reproduction
volume
133
issue
2
pages
517 - 530
publisher
BioScientifica Ltd.
external identifiers
  • wos:000244680500018
  • scopus:33847675040
ISSN
1470-1626
DOI
10.1530/10.1530/REP-06-0195
language
English
LU publication?
yes
id
b1fbfe95-81c7-491e-aa88-1dc24e3403f8 (old id 670952)
date added to LUP
2007-12-17 10:38:38
date last changed
2017-01-01 06:41:27
@article{b1fbfe95-81c7-491e-aa88-1dc24e3403f8,
  abstract     = {Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population.},
  author       = {Long, Manhai and Stronati, Alessandra and Bizzaro, Davide and Kruger, Tanja and Manicardi, Gian-Carlo and Hjelmborg, Philip S. and Spano, Marcello and Giwercman, Aleksander and Toft, Gunnar and Bonde, Jens Peter and Bonefeld-Jorgensen, Eva C.},
  issn         = {1470-1626},
  language     = {eng},
  number       = {2},
  pages        = {517--530},
  publisher    = {BioScientifica Ltd.},
  series       = {Reproduction},
  title        = {Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations},
  url          = {http://dx.doi.org/10.1530/10.1530/REP-06-0195},
  volume       = {133},
  year         = {2007},
}