Advanced

Application of optical biosensors in small-molecule screening activities

Geschwindner, Stefan ; Carlsson, Johan F and Knecht, Wolfgang LU (2012) In Sensors (Basel, Switzerland) 12(4). p.4311-4323
Abstract

The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay... (More)

The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay formats on these platforms. A particular focus will be on the specific advantages of the inhibition in solution assay (ISA) format in contrast to traditional direct binding assays (DBA). Furthermore we will discuss different application areas for both fluidic as well as plate-based biosensor systems by considering the individual strength of the platforms.

(Less)
Please use this url to cite or link to this publication:
author
; and
publishing date
type
Contribution to journal
publication status
published
keywords
Biosensing Techniques, Optics and Photonics, Surface Plasmon Resonance
in
Sensors (Basel, Switzerland)
volume
12
issue
4
pages
4311 - 4323
publisher
MDPI AG
external identifiers
  • scopus:84860124363
  • pmid:22666031
ISSN
1424-8220
DOI
10.3390/s120404311
language
English
LU publication?
no
id
671b2ac7-5492-4b6b-bd99-dd78060501b3
date added to LUP
2020-07-17 14:08:50
date last changed
2020-07-22 10:30:20
@article{671b2ac7-5492-4b6b-bd99-dd78060501b3,
  abstract     = {<p>The last two decades have seen remarkable progress and improvements in optical biosensor systems such that those are currently seen as an important and value-adding component of modern drug screening activities. In particular the introduction of microplate-based biosensor systems holds the promise to match the required throughput without compromising on data quality thus representing a sought-after complement to traditional fluidic systems. This article aims to highlight the application of the two most prominent optical biosensor technologies, namely surface plasmon resonance (SPR) and optical waveguide grating (OWG), in small-molecule screening and will present, review and discuss the advantages and disadvantages of different assay formats on these platforms. A particular focus will be on the specific advantages of the inhibition in solution assay (ISA) format in contrast to traditional direct binding assays (DBA). Furthermore we will discuss different application areas for both fluidic as well as plate-based biosensor systems by considering the individual strength of the platforms.</p>},
  author       = {Geschwindner, Stefan and Carlsson, Johan F and Knecht, Wolfgang},
  issn         = {1424-8220},
  language     = {eng},
  month        = {03},
  number       = {4},
  pages        = {4311--4323},
  publisher    = {MDPI AG},
  series       = {Sensors (Basel, Switzerland)},
  title        = {Application of optical biosensors in small-molecule screening activities},
  url          = {http://dx.doi.org/10.3390/s120404311},
  doi          = {10.3390/s120404311},
  volume       = {12},
  year         = {2012},
}