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Quantification of HIV-2 DNA in Whole Blood

Szojka, Zsófia LU ; Karlson, Sara LU orcid ; Jansson, Marianne LU and Medstrand, Patrik LU orcid (2019) In Bio-protocol 9(20).
Abstract

Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, quantification of cellular DNA load may constitute an alternative method for monitoring ART efficacy. Moreover, sensitive HIV-2 DNA quantification protocols are also important for the characterization of the HIV-2 reservoirs, and ultimately for the development of HIV-2 cure strategies. We have developed a sensitive and robust HIV-2 DNA quantification protocol based on whole blood as DNA source,... (More)

Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, quantification of cellular DNA load may constitute an alternative method for monitoring ART efficacy. Moreover, sensitive HIV-2 DNA quantification protocols are also important for the characterization of the HIV-2 reservoirs, and ultimately for the development of HIV-2 cure strategies. We have developed a sensitive and robust HIV-2 DNA quantification protocol based on whole blood as DNA source, including normalization of leukocyte cell numbers using parallel quantification of the single copy porphobilinogen deaminase gene. The specificity and sensitivity of the assay was 100%. The limit of detection was 1 copy and limit of quantification was 5 copies. When applying this protocol to HIV-2 infected, it was found that HIV-2 viral DNA was detectable in individuals in whom viral RNA was undetectable or under quantification level. Thus, this method provides a sensitive approach to HIV-2 DNA viral quantification from whole blood of HIV-2 infected patients.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bio-protocol
volume
9
issue
20
article number
e3404
publisher
Bio-protocol LLC
external identifiers
  • pmid:33654905
  • scopus:85129259309
ISSN
2331-8325
DOI
10.21769/BioProtoc.3404
language
English
LU publication?
yes
additional info
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
id
671f6da9-b604-4873-bcbc-1e5c3e8a425e
date added to LUP
2024-09-17 10:59:54
date last changed
2024-09-18 04:01:32
@article{671f6da9-b604-4873-bcbc-1e5c3e8a425e,
  abstract     = {{<p>Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, quantification of cellular DNA load may constitute an alternative method for monitoring ART efficacy. Moreover, sensitive HIV-2 DNA quantification protocols are also important for the characterization of the HIV-2 reservoirs, and ultimately for the development of HIV-2 cure strategies. We have developed a sensitive and robust HIV-2 DNA quantification protocol based on whole blood as DNA source, including normalization of leukocyte cell numbers using parallel quantification of the single copy porphobilinogen deaminase gene. The specificity and sensitivity of the assay was 100%. The limit of detection was 1 copy and limit of quantification was 5 copies. When applying this protocol to HIV-2 infected, it was found that HIV-2 viral DNA was detectable in individuals in whom viral RNA was undetectable or under quantification level. Thus, this method provides a sensitive approach to HIV-2 DNA viral quantification from whole blood of HIV-2 infected patients.</p>}},
  author       = {{Szojka, Zsófia and Karlson, Sara and Jansson, Marianne and Medstrand, Patrik}},
  issn         = {{2331-8325}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{20}},
  publisher    = {{Bio-protocol LLC}},
  series       = {{Bio-protocol}},
  title        = {{Quantification of HIV-2 DNA in Whole Blood}},
  url          = {{http://dx.doi.org/10.21769/BioProtoc.3404}},
  doi          = {{10.21769/BioProtoc.3404}},
  volume       = {{9}},
  year         = {{2019}},
}