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Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde

Khlupova, Maria; Kuznetsov, Boris; Demkiv, Olha; Gonchar, Mykhailo; Csöregi, Elisabeth LU and Shleev, Sergey LU (2007) In Talanta 71(2). p.934-940
Abstract
Intact and permeabilized yeast cells were tested as the biorecognition elements for amperometric assay of formaldehyde (FA). For this aim, the mutant G 105 (gcr1 catX) of the methylotrophic yeast Hansenula polymorpha with a high activity of AOX was chosen. Different approaches were used for monitoring FA-dependent cell response including analysis of their oxygen consumption rate by the use of a Clark electrode, as well as assay of oxidation of redox mediator at a screen-printed platinum electrode covered by cells entrapped in Ca-alginate gel. It was shown that oxygen consumption rate of permeabilized cells reached its saturation at 4 mM of FA (23 degrees C). The detection limit was found to be 0.27 mM. In the presence of redox mediator... (More)
Intact and permeabilized yeast cells were tested as the biorecognition elements for amperometric assay of formaldehyde (FA). For this aim, the mutant G 105 (gcr1 catX) of the methylotrophic yeast Hansenula polymorpha with a high activity of AOX was chosen. Different approaches were used for monitoring FA-dependent cell response including analysis of their oxygen consumption rate by the use of a Clark electrode, as well as assay of oxidation of redox mediator at a screen-printed platinum electrode covered by cells entrapped in Ca-alginate gel. It was shown that oxygen consumption rate of permeabilized cells reached its saturation at 4 mM of FA (23 degrees C). The detection limit was found to be 0.27 mM. In the presence of redox mediator 2,6-dichlorophenolindophenol (DCIP), the screen-printed platinum band electrode covered by permeabilized cells did not show any current output to FA. In contrast, well-pronounced amperometric response to FA was observed in the case of intact yeast cells in the presence of DCIR It was shown that current output reached its maximum at 7 mM concentration of FA. The detection limit was found to be 0.74 mM. Obviously, it is necessary to perform a directed genetic engineering of the yeast cells to improve their bioanalytical characteristics in the corresponding biosensors. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
intact and permeabilized cells, mediator, redox, amperometry, biosensor, bioselective analysis, formaldehyde, yeast Hansenula polymorpha
in
Talanta
volume
71
issue
2
pages
934 - 940
publisher
Elsevier
external identifiers
  • wos:000244369600064
  • scopus:33846245976
ISSN
1873-3573
DOI
10.1016/j.talanta.2006.05.069
language
English
LU publication?
yes
id
b39ce422-975d-4d5e-a0f1-77b82768fed0 (old id 672903)
date added to LUP
2007-12-13 16:36:25
date last changed
2017-01-01 06:40:01
@article{b39ce422-975d-4d5e-a0f1-77b82768fed0,
  abstract     = {Intact and permeabilized yeast cells were tested as the biorecognition elements for amperometric assay of formaldehyde (FA). For this aim, the mutant G 105 (gcr1 catX) of the methylotrophic yeast Hansenula polymorpha with a high activity of AOX was chosen. Different approaches were used for monitoring FA-dependent cell response including analysis of their oxygen consumption rate by the use of a Clark electrode, as well as assay of oxidation of redox mediator at a screen-printed platinum electrode covered by cells entrapped in Ca-alginate gel. It was shown that oxygen consumption rate of permeabilized cells reached its saturation at 4 mM of FA (23 degrees C). The detection limit was found to be 0.27 mM. In the presence of redox mediator 2,6-dichlorophenolindophenol (DCIP), the screen-printed platinum band electrode covered by permeabilized cells did not show any current output to FA. In contrast, well-pronounced amperometric response to FA was observed in the case of intact yeast cells in the presence of DCIR It was shown that current output reached its maximum at 7 mM concentration of FA. The detection limit was found to be 0.74 mM. Obviously, it is necessary to perform a directed genetic engineering of the yeast cells to improve their bioanalytical characteristics in the corresponding biosensors. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Khlupova, Maria and Kuznetsov, Boris and Demkiv, Olha and Gonchar, Mykhailo and Csöregi, Elisabeth and Shleev, Sergey},
  issn         = {1873-3573},
  keyword      = {intact and permeabilized cells,mediator,redox,amperometry,biosensor,bioselective analysis,formaldehyde,yeast Hansenula polymorpha},
  language     = {eng},
  number       = {2},
  pages        = {934--940},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde},
  url          = {http://dx.doi.org/10.1016/j.talanta.2006.05.069},
  volume       = {71},
  year         = {2007},
}