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Development of a plasma panel test for detection of human myocardial proteins by capillary immunoassay

Torabi, Fereidon; Far, Hamid Reza Mobini; Danielsson, Bengt LU and Khayyami, Masoud (2007) In Biosensors & Bioelectronics 22(7). p.1218-1223
Abstract
A chemiluminescence inummoassay for the detection of four heart marker proteins: myoglobin, creatine kinase mb [CKmb], troponin I [Tnl], and fatty acid-binding protein [FABP], was designed. The inummoassay was based on enzyme-linked immunosorbent assay [ELISA] and antibodies immobilized in glass capillaries pre-treated with 3-aminopropyltriethoxysilane. The protein bound to the antibody was detected by using an antiprotein-horseradish peroxidase [HR-P] conjugate. The reaction of the HRP with luminal and hydrogen peroxide-based substrate generated the chemiluminescence and a photodiode detector was used to measure the light intensity. The same assay protocol was used to detect all four proteins. Ultrasound waves were used to improve the... (More)
A chemiluminescence inummoassay for the detection of four heart marker proteins: myoglobin, creatine kinase mb [CKmb], troponin I [Tnl], and fatty acid-binding protein [FABP], was designed. The inummoassay was based on enzyme-linked immunosorbent assay [ELISA] and antibodies immobilized in glass capillaries pre-treated with 3-aminopropyltriethoxysilane. The protein bound to the antibody was detected by using an antiprotein-horseradish peroxidase [HR-P] conjugate. The reaction of the HRP with luminal and hydrogen peroxide-based substrate generated the chemiluminescence and a photodiode detector was used to measure the light intensity. The same assay protocol was used to detect all four proteins. Ultrasound waves were used to improve the silanization of glass and the antibody immobilization process. The optimization of the duration and intensity of the ultrasound was performed for the myoglobin assay. Ultrasound improved the silanization procedure and the capillaries gave an approximately 2.5 times greater ELISA response. Ultrasound also improved the sensitivity by approximately 100% when monoclonal antibody was immobilized on a glass capillary. Calibration curves corresponding to analyte concentrations ranging from 2.4 to 2400 ng/ml in plasma samples were recorded. The detection limits were in the region of 1.2 myoglobin, 0.6 CKmb, 5.6 TnI, and 4 ng/ml FABP in plasma with a coefficient of variation of 3-9.9%. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ultrasound, chemiluminescent ELISA, creatine kinase mb, myoglobin, fatty acid-binding protein, troponin I
in
Biosensors & Bioelectronics
volume
22
issue
7
pages
1218 - 1223
publisher
Elsevier
external identifiers
  • wos:000244385400005
  • scopus:33846500348
ISSN
1873-4235
DOI
10.1016/j.bios.2006.04.030
language
English
LU publication?
yes
id
65a4a06c-f804-45ca-816e-8f3d421dcdd8 (old id 673966)
date added to LUP
2007-12-19 10:34:49
date last changed
2017-08-13 04:22:48
@article{65a4a06c-f804-45ca-816e-8f3d421dcdd8,
  abstract     = {A chemiluminescence inummoassay for the detection of four heart marker proteins: myoglobin, creatine kinase mb [CKmb], troponin I [Tnl], and fatty acid-binding protein [FABP], was designed. The inummoassay was based on enzyme-linked immunosorbent assay [ELISA] and antibodies immobilized in glass capillaries pre-treated with 3-aminopropyltriethoxysilane. The protein bound to the antibody was detected by using an antiprotein-horseradish peroxidase [HR-P] conjugate. The reaction of the HRP with luminal and hydrogen peroxide-based substrate generated the chemiluminescence and a photodiode detector was used to measure the light intensity. The same assay protocol was used to detect all four proteins. Ultrasound waves were used to improve the silanization of glass and the antibody immobilization process. The optimization of the duration and intensity of the ultrasound was performed for the myoglobin assay. Ultrasound improved the silanization procedure and the capillaries gave an approximately 2.5 times greater ELISA response. Ultrasound also improved the sensitivity by approximately 100% when monoclonal antibody was immobilized on a glass capillary. Calibration curves corresponding to analyte concentrations ranging from 2.4 to 2400 ng/ml in plasma samples were recorded. The detection limits were in the region of 1.2 myoglobin, 0.6 CKmb, 5.6 TnI, and 4 ng/ml FABP in plasma with a coefficient of variation of 3-9.9%. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Torabi, Fereidon and Far, Hamid Reza Mobini and Danielsson, Bengt and Khayyami, Masoud},
  issn         = {1873-4235},
  keyword      = {ultrasound,chemiluminescent ELISA,creatine kinase mb,myoglobin,fatty acid-binding protein,troponin I},
  language     = {eng},
  number       = {7},
  pages        = {1218--1223},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Development of a plasma panel test for detection of human myocardial proteins by capillary immunoassay},
  url          = {http://dx.doi.org/10.1016/j.bios.2006.04.030},
  volume       = {22},
  year         = {2007},
}