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Fed-batch cultivation of Saccharomyces cerevisiae on lignocellulosic hydrolyzate

Petersson, Anneli and Lidén, Gunnar LU (2007) In Biotechnology Letters 29(2). p.219-225
Abstract
Saccharomyces cerevisiae grows very poorly in dilute acid lignocellulosic hydrolyzate during the anaerobic fermentation for fuel ethanol production. However, yeast cells grown aerobically on the hydrolyzate have increased tolerance for the hydrolyzate. Cultivation of yeast on part of the hydrolyzate has therefore the potential of enabling increased ethanol productivity in the fermentation of the hydrolyzate. To evaluate the ability of the yeast to grow in the hydrolyzate, fed-batch cultivations were run using the ethanol concentration as input variable to control the feed-rate. The yeast then grew in an undetoxified hydrolyzate with a specific growth rate of 0.19 h(-1) by controlling the ethanol concentration at a low level during the... (More)
Saccharomyces cerevisiae grows very poorly in dilute acid lignocellulosic hydrolyzate during the anaerobic fermentation for fuel ethanol production. However, yeast cells grown aerobically on the hydrolyzate have increased tolerance for the hydrolyzate. Cultivation of yeast on part of the hydrolyzate has therefore the potential of enabling increased ethanol productivity in the fermentation of the hydrolyzate. To evaluate the ability of the yeast to grow in the hydrolyzate, fed-batch cultivations were run using the ethanol concentration as input variable to control the feed-rate. The yeast then grew in an undetoxified hydrolyzate with a specific growth rate of 0.19 h(-1) by controlling the ethanol concentration at a low level during the cultivation. However, the biomass yield was lower for the cultivation on hydrolyzate compared to synthetic media: with an ethanol set-point of 0.25 g/l the yield was 0.46 g/g on the hydrolyzate, compared to 0.52 g/g for synthetic media. The main reason for the difference was not the ethanol production per se, but a significant production of glycerol at a high specific growth rate. The glycerol production may be attributed to an insufficient respiratory capacity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
yeast production, Saccharomyces cerevisiae, dilute acid hydrolyzate, fed-batch control
in
Biotechnology Letters
volume
29
issue
2
pages
219 - 225
publisher
Springer
external identifiers
  • wos:000244292900005
  • scopus:33846177942
ISSN
1573-6776
DOI
10.1007/s10529-006-9227-z
language
English
LU publication?
yes
id
c1427547-80ac-4184-96e0-93852475614b (old id 674316)
date added to LUP
2007-12-14 08:24:42
date last changed
2017-08-27 05:27:11
@article{c1427547-80ac-4184-96e0-93852475614b,
  abstract     = {Saccharomyces cerevisiae grows very poorly in dilute acid lignocellulosic hydrolyzate during the anaerobic fermentation for fuel ethanol production. However, yeast cells grown aerobically on the hydrolyzate have increased tolerance for the hydrolyzate. Cultivation of yeast on part of the hydrolyzate has therefore the potential of enabling increased ethanol productivity in the fermentation of the hydrolyzate. To evaluate the ability of the yeast to grow in the hydrolyzate, fed-batch cultivations were run using the ethanol concentration as input variable to control the feed-rate. The yeast then grew in an undetoxified hydrolyzate with a specific growth rate of 0.19 h(-1) by controlling the ethanol concentration at a low level during the cultivation. However, the biomass yield was lower for the cultivation on hydrolyzate compared to synthetic media: with an ethanol set-point of 0.25 g/l the yield was 0.46 g/g on the hydrolyzate, compared to 0.52 g/g for synthetic media. The main reason for the difference was not the ethanol production per se, but a significant production of glycerol at a high specific growth rate. The glycerol production may be attributed to an insufficient respiratory capacity.},
  author       = {Petersson, Anneli and Lidén, Gunnar},
  issn         = {1573-6776},
  keyword      = {yeast production,Saccharomyces cerevisiae,dilute acid hydrolyzate,fed-batch control},
  language     = {eng},
  number       = {2},
  pages        = {219--225},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Fed-batch cultivation of Saccharomyces cerevisiae on lignocellulosic hydrolyzate},
  url          = {http://dx.doi.org/10.1007/s10529-006-9227-z},
  volume       = {29},
  year         = {2007},
}