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Photoelectrocatalytic oxidation of NADH with electropolymerized Toluidine Blue O

Dilgin, Yusuf; Gorton, Lo LU and Nisli, Gurel (2007) In Electroanalysis 19(2-3). p.286-293
Abstract
A poly(Toluidine Blue O) (poly-TBO) modified electrode was successfully prepared by repeated sweeping the applied potential from -0.6 to + 0.8 V (vs. SCE) on a glassy carbon electrode (GCE) in borate buffer solution at pH 9.1 containing 0.1 M NaNO3 and 0.4 mM Toluidine Blue 0 (TBO). The poly-TBO modified GCE shows electrocatalytic activity toward NADH oxidation in phosphate buffer solution at pH 7.0, with an overpotential of ca. 350 mV lower than that at the bare electrode. The photoelectrocatalytic oxidation of NADH at this electrode was also successfully investigated by using cyclic voltammetry and amperometry at constant potential. When the modified electrode surface was irradiated with a 250 W halogen lamp, a photoelectrocatalytic... (More)
A poly(Toluidine Blue O) (poly-TBO) modified electrode was successfully prepared by repeated sweeping the applied potential from -0.6 to + 0.8 V (vs. SCE) on a glassy carbon electrode (GCE) in borate buffer solution at pH 9.1 containing 0.1 M NaNO3 and 0.4 mM Toluidine Blue 0 (TBO). The poly-TBO modified GCE shows electrocatalytic activity toward NADH oxidation in phosphate buffer solution at pH 7.0, with an overpotential of ca. 350 mV lower than that at the bare electrode. The photoelectrocatalytic oxidation of NADH at this electrode was also successfully investigated by using cyclic voltammetry and amperometry at constant potential. When the modified electrode surface was irradiated with a 250 W halogen lamp, a photoelectrocatalytic effect was observed for NADH oxidation and the current was increased about 2.2 times. The applied potential was selected at +100mV for amperometric and photoamperometric detection of NADH. A linear calibration graph for NADH was obtained in the range between 1.0 x 10(-5) and 1.0 x 10(-3) M and between 5.0 x 10(-6) and 1.0 X 10(-3) M for amperometric and photoamperometric studies, respectively. The effect of some interfering compounds, such as ascorbic acid and dopamine on the electrocatalytic and photoclectrocatalytic oxidation of NADH was tested. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Toluidine Blue, amperometric sensor, modified glassy carbon electrode, O, NADH
in
Electroanalysis
volume
19
issue
2-3
pages
286 - 293
publisher
John Wiley & Sons
external identifiers
  • wos:000244220700024
  • scopus:34547594775
ISSN
1040-0397
DOI
10.1002/elan.200603730
language
English
LU publication?
yes
id
0218974b-1fc3-464e-ad5d-9e6548ca36df (old id 674680)
date added to LUP
2007-12-10 14:50:48
date last changed
2017-09-24 04:32:44
@article{0218974b-1fc3-464e-ad5d-9e6548ca36df,
  abstract     = {A poly(Toluidine Blue O) (poly-TBO) modified electrode was successfully prepared by repeated sweeping the applied potential from -0.6 to + 0.8 V (vs. SCE) on a glassy carbon electrode (GCE) in borate buffer solution at pH 9.1 containing 0.1 M NaNO3 and 0.4 mM Toluidine Blue 0 (TBO). The poly-TBO modified GCE shows electrocatalytic activity toward NADH oxidation in phosphate buffer solution at pH 7.0, with an overpotential of ca. 350 mV lower than that at the bare electrode. The photoelectrocatalytic oxidation of NADH at this electrode was also successfully investigated by using cyclic voltammetry and amperometry at constant potential. When the modified electrode surface was irradiated with a 250 W halogen lamp, a photoelectrocatalytic effect was observed for NADH oxidation and the current was increased about 2.2 times. The applied potential was selected at +100mV for amperometric and photoamperometric detection of NADH. A linear calibration graph for NADH was obtained in the range between 1.0 x 10(-5) and 1.0 x 10(-3) M and between 5.0 x 10(-6) and 1.0 X 10(-3) M for amperometric and photoamperometric studies, respectively. The effect of some interfering compounds, such as ascorbic acid and dopamine on the electrocatalytic and photoclectrocatalytic oxidation of NADH was tested.},
  author       = {Dilgin, Yusuf and Gorton, Lo and Nisli, Gurel},
  issn         = {1040-0397},
  keyword      = {Toluidine Blue,amperometric sensor,modified glassy carbon electrode,O,NADH},
  language     = {eng},
  number       = {2-3},
  pages        = {286--293},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Photoelectrocatalytic oxidation of NADH with electropolymerized Toluidine Blue O},
  url          = {http://dx.doi.org/10.1002/elan.200603730},
  volume       = {19},
  year         = {2007},
}