Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes
(2007) In Electroanalysis 19(2-3). p.294-302- Abstract
- Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A.... (More)
- Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/674684
- author
- Tasca, Federico LU ; Timur, Suna ; Ludwig, Roland ; Haltrich, Dietmar ; Volc, Jindrich ; Antiochia, Riccarda and Gorton, Lo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell anode, biofuel, nonselective sugar oxidizing enzyme, flow injection analysis
- in
- Electroanalysis
- volume
- 19
- issue
- 2-3
- pages
- 294 - 302
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000244220700025
- scopus:33846442623
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.200603740
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 9ce597b4-6503-4fd8-84d4-3043dc2666a1 (old id 674684)
- date added to LUP
- 2016-04-01 16:19:11
- date last changed
- 2022-04-22 21:14:07
@article{9ce597b4-6503-4fd8-84d4-3043dc2666a1, abstract = {{Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars.}}, author = {{Tasca, Federico and Timur, Suna and Ludwig, Roland and Haltrich, Dietmar and Volc, Jindrich and Antiochia, Riccarda and Gorton, Lo}}, issn = {{1040-0397}}, keywords = {{cell anode; biofuel; nonselective sugar oxidizing enzyme; flow injection analysis}}, language = {{eng}}, number = {{2-3}}, pages = {{294--302}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electroanalysis}}, title = {{Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes}}, url = {{http://dx.doi.org/10.1002/elan.200603740}}, doi = {{10.1002/elan.200603740}}, volume = {{19}}, year = {{2007}}, }