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Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes

Tasca, Federico LU ; Timur, Suna; Ludwig, Roland; Haltrich, Dietmar; Volc, Jindrich; Antiochia, Riccarda and Gorton, Lo LU (2007) In Electroanalysis 19(2-3). p.294-302
Abstract
Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A.... (More)
Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell anode, biofuel, nonselective sugar oxidizing enzyme, flow injection analysis
in
Electroanalysis
volume
19
issue
2-3
pages
294 - 302
publisher
John Wiley & Sons
external identifiers
  • wos:000244220700025
  • scopus:33846442623
ISSN
1040-0397
DOI
10.1002/elan.200603740
language
English
LU publication?
yes
id
9ce597b4-6503-4fd8-84d4-3043dc2666a1 (old id 674684)
date added to LUP
2008-01-03 09:16:51
date last changed
2017-02-12 04:08:19
@article{9ce597b4-6503-4fd8-84d4-3043dc2666a1,
  abstract     = {Electrical wiring of different types of pyranose oxidase (P20) (fungal wild type, recombinant wild type with a hexahistidine tag, mutant form E542K with a hexa-histidine tag) from Trametes multicolor, and recombinant P20 from Coriolus sp. overexpressed in Escherichia coli as well as of pyranose dehydrogenase (PDH) from Agaricus meleagris and Agaricus xanthoderma with an osmium redox polymer (poly(1-vinylimidazole)(12)-[Os(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl2+/+) on graphite electrodes was carried out. After optimization studies using glucose as substrate, the biosensors, which showed the best characteristics in terms of linear range, detection limit and sensitivity were selected, viz. wild type P20 from T multicolor and PDH from A. meleagris. These two enzymes were used and investigated for their selectivity for a number of different sugars.},
  author       = {Tasca, Federico and Timur, Suna and Ludwig, Roland and Haltrich, Dietmar and Volc, Jindrich and Antiochia, Riccarda and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {cell anode,biofuel,nonselective sugar oxidizing enzyme,flow injection analysis},
  language     = {eng},
  number       = {2-3},
  pages        = {294--302},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes},
  url          = {http://dx.doi.org/10.1002/elan.200603740},
  volume       = {19},
  year         = {2007},
}