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Stability of immobilized Candida antarctica lipase B during chemo-enzymatic epoxidation of fatty acids

Törnvall, Ulrika LU ; Orellana Coca Åkerman, Cecilia LU ; Hatti-Kaul, Rajni LU and Adlercreutz, Dietlind LU (2007) In Enzyme and Microbial Technology 40(3). p.447-451
Abstract
The parameters affecting the lipase activity and operational lifetime during chemo-enzymatic epoxidation of fatty acids were investigated. Immobilized Candida antarctica lipase B (Novozym (R) 435) was incubated in the presence of various reaction components (i.e. toluene, water, H2O2, oleic acid, perpalmitic acid, and epoxystearic acid, respectively) at temperatures between 20 and 60 degrees C followed by measurement of residual enzyme activity. Epoxystearic acid was shown to slightly inactivate the enzyme at 50 degrees C, while oleic acid and perpalmitic acid did not. No deactivation of the enzyme was observed in presence of toluene/water mixture within 48 h at 20-60 degrees C. In the presence of 6-12 M hydrogen peroxide, the enzyme was... (More)
The parameters affecting the lipase activity and operational lifetime during chemo-enzymatic epoxidation of fatty acids were investigated. Immobilized Candida antarctica lipase B (Novozym (R) 435) was incubated in the presence of various reaction components (i.e. toluene, water, H2O2, oleic acid, perpalmitic acid, and epoxystearic acid, respectively) at temperatures between 20 and 60 degrees C followed by measurement of residual enzyme activity. Epoxystearic acid was shown to slightly inactivate the enzyme at 50 degrees C, while oleic acid and perpalmitic acid did not. No deactivation of the enzyme was observed in presence of toluene/water mixture within 48 h at 20-60 degrees C. In the presence of 6-12 M hydrogen peroxide, the enzyme was rather stable at 20 degrees C, while at 60 degrees C the enzyme lost activity rapidly, with the rate of deactivation increasing with increasing hydrogen peroxide concentration. These results imply that temperature control and careful dosage of hydrogen peroxide would be essential in an industrial chemo-enzymatic process. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oxidation, lipase deactivation, epoxidation, solvent-free process, hydrogen, peracid, peroxide
in
Enzyme and Microbial Technology
volume
40
issue
3
pages
447 - 451
publisher
Elsevier
external identifiers
  • wos:000244072500013
  • scopus:33846081609
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2006.07.019
language
English
LU publication?
yes
id
61d1720d-bf18-4e33-8534-ecbf74a37e35 (old id 675236)
date added to LUP
2008-01-02 14:25:05
date last changed
2017-11-12 03:22:50
@article{61d1720d-bf18-4e33-8534-ecbf74a37e35,
  abstract     = {The parameters affecting the lipase activity and operational lifetime during chemo-enzymatic epoxidation of fatty acids were investigated. Immobilized Candida antarctica lipase B (Novozym (R) 435) was incubated in the presence of various reaction components (i.e. toluene, water, H2O2, oleic acid, perpalmitic acid, and epoxystearic acid, respectively) at temperatures between 20 and 60 degrees C followed by measurement of residual enzyme activity. Epoxystearic acid was shown to slightly inactivate the enzyme at 50 degrees C, while oleic acid and perpalmitic acid did not. No deactivation of the enzyme was observed in presence of toluene/water mixture within 48 h at 20-60 degrees C. In the presence of 6-12 M hydrogen peroxide, the enzyme was rather stable at 20 degrees C, while at 60 degrees C the enzyme lost activity rapidly, with the rate of deactivation increasing with increasing hydrogen peroxide concentration. These results imply that temperature control and careful dosage of hydrogen peroxide would be essential in an industrial chemo-enzymatic process. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Törnvall, Ulrika and Orellana Coca Åkerman, Cecilia and Hatti-Kaul, Rajni and Adlercreutz, Dietlind},
  issn         = {0141-0229},
  keyword      = {oxidation,lipase deactivation,epoxidation,solvent-free process,hydrogen,peracid,peroxide},
  language     = {eng},
  number       = {3},
  pages        = {447--451},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Stability of immobilized Candida antarctica lipase B during chemo-enzymatic epoxidation of fatty acids},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2006.07.019},
  volume       = {40},
  year         = {2007},
}