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Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: The control of particle size suitable for different analytical applications

Yoshimatsu, Keiichi LU ; Reimhult, Kristina; Krozer, Anatol; Mosbach, Klaus LU ; Sode, Koji and Ye, Lei LU (2007) In Analytica Chimica Acta 584(1). p.112-121
Abstract
Molecularly imprinted polymers (MIPs) are being increasingly used as selective adsorbents in different analytical applications. To satisfy the different application purposes, MEN with well controlled physical forms in different size ranges are highly desirable. For examples, MIP nanoparticles are very suitable to be used to develop binding assays and for microfluidic separations, whereas MIP beads with diameter of 1.5-3 mu m can be more appropriate to use in new analytical liquid chromatography systems. Previous studies have demonstrated that imprinted microspheres and nanoparticles can be synthesized using a simple precipitation polymerization method. Despite that the synthetic method is straightforward, the final particle size obtained... (More)
Molecularly imprinted polymers (MIPs) are being increasingly used as selective adsorbents in different analytical applications. To satisfy the different application purposes, MEN with well controlled physical forms in different size ranges are highly desirable. For examples, MIP nanoparticles are very suitable to be used to develop binding assays and for microfluidic separations, whereas MIP beads with diameter of 1.5-3 mu m can be more appropriate to use in new analytical liquid chromatography systems. Previous studies have demonstrated that imprinted microspheres and nanoparticles can be synthesized using a simple precipitation polymerization method. Despite that the synthetic method is straightforward, the final particle size obtained has been difficult to adjust for a given template. In this work, we initiated to study new synthetic conditions to obtain MIP beads with controllable size in the nano- to micro-meter range, using racemic propranolol as a model template. Varying the composition of the cross-linking monomer allowed the particle size of the MIP beads to be altered in the range of 130 nm to 2.4 mu m, whereas the favorable binding property of the imprinted beads remained intact. The chiral recognition sites were further characterized with equilibrium binding analysis using tritium-labeled (S)-propranolol as a tracer. In general, the imprinted sites displayed a high chiral selectivity: the apparent affinity of the (S)-imprinted sites for (S)-propranolol was 20 times that of for (R)-propranolol. Compared to previously reported irregular particles, the chiral selectivity of competitive radioligand binding assays developed from the present imprinted beads has been increased by six to seven folds in an optimized aqueous solvent. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
assay, propranolol, molecular imprinting, precipitation polymerization, nanoparticles
in
Analytica Chimica Acta
volume
584
issue
1
pages
112 - 121
publisher
Elsevier
external identifiers
  • wos:000244020700017
  • scopus:33846308791
ISSN
1873-4324
DOI
10.1016/j.aca.2006.11.004
language
English
LU publication?
yes
id
6eb958ca-4363-4fbf-8edf-012bec8872f6 (old id 675342)
date added to LUP
2008-01-03 11:14:34
date last changed
2017-10-22 04:30:54
@article{6eb958ca-4363-4fbf-8edf-012bec8872f6,
  abstract     = {Molecularly imprinted polymers (MIPs) are being increasingly used as selective adsorbents in different analytical applications. To satisfy the different application purposes, MEN with well controlled physical forms in different size ranges are highly desirable. For examples, MIP nanoparticles are very suitable to be used to develop binding assays and for microfluidic separations, whereas MIP beads with diameter of 1.5-3 mu m can be more appropriate to use in new analytical liquid chromatography systems. Previous studies have demonstrated that imprinted microspheres and nanoparticles can be synthesized using a simple precipitation polymerization method. Despite that the synthetic method is straightforward, the final particle size obtained has been difficult to adjust for a given template. In this work, we initiated to study new synthetic conditions to obtain MIP beads with controllable size in the nano- to micro-meter range, using racemic propranolol as a model template. Varying the composition of the cross-linking monomer allowed the particle size of the MIP beads to be altered in the range of 130 nm to 2.4 mu m, whereas the favorable binding property of the imprinted beads remained intact. The chiral recognition sites were further characterized with equilibrium binding analysis using tritium-labeled (S)-propranolol as a tracer. In general, the imprinted sites displayed a high chiral selectivity: the apparent affinity of the (S)-imprinted sites for (S)-propranolol was 20 times that of for (R)-propranolol. Compared to previously reported irregular particles, the chiral selectivity of competitive radioligand binding assays developed from the present imprinted beads has been increased by six to seven folds in an optimized aqueous solvent. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Yoshimatsu, Keiichi and Reimhult, Kristina and Krozer, Anatol and Mosbach, Klaus and Sode, Koji and Ye, Lei},
  issn         = {1873-4324},
  keyword      = {assay,propranolol,molecular imprinting,precipitation polymerization,nanoparticles},
  language     = {eng},
  number       = {1},
  pages        = {112--121},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: The control of particle size suitable for different analytical applications},
  url          = {http://dx.doi.org/10.1016/j.aca.2006.11.004},
  volume       = {584},
  year         = {2007},
}