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NADH screen-printed electrodes modified with zirconium phosphate, Meldola blue, and Reinecke salt. Application to the detection of glycerol by FIA

Radoi, A.; Compagnone, D.; Batic, M.; Klincar, J.; Gorton, Lo LU and Palleschi, G. (2007) In Analytical and Bioanalytical Chemistry 387(3). p.1049-1058
Abstract
Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10(-4) mol L-1 NADH, %RSD=4.2, n=90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag-AgCl), flow rate (0.30 mL min(-1)) and pH-dependence... (More)
Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10(-4) mol L-1 NADH, %RSD=4.2, n=90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag-AgCl), flow rate (0.30 mL min(-1)) and pH-dependence (range 4.0-10.0) were evaluated and optimized. A glycerol biosensor, prepared by immobilizing glycerol dehydrogenase (GDH) on the working electrode area of a bMBZrPRs-SPE, was also assembled. The biosensor was most stable at pH 8.5 (%RSD=5.6, n=70, 0.25 mmol L-1 glycerol). The detection and quantification limits were 2.8x10(-6) and 9.4x10(-6) mol L-1, respectively, and the linear working range was between 1.0x10(-5) and 1.0x10(-4) mol L-1. To assess the effect of interferences, and recovery by the probe we analyzed samples taken during fermentation of chemically defined grape juice medium and compared the results with those obtained by HPLC. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
NADH, Reinecke salt, zirconium phosphate, meldola blue, glycerol, biosensor
in
Analytical and Bioanalytical Chemistry
volume
387
issue
3
pages
1049 - 1058
publisher
Springer
external identifiers
  • wos:000243815500033
  • scopus:33846592203
ISSN
1618-2642
DOI
10.1007/s00216-006-0975-3
language
English
LU publication?
yes
id
98148236-b077-4880-82c4-1c1ee8a51b69 (old id 676456)
date added to LUP
2007-12-19 09:36:05
date last changed
2017-02-12 03:33:00
@article{98148236-b077-4880-82c4-1c1ee8a51b69,
  abstract     = {Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10(-4) mol L-1 NADH, %RSD=4.2, n=90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag-AgCl), flow rate (0.30 mL min(-1)) and pH-dependence (range 4.0-10.0) were evaluated and optimized. A glycerol biosensor, prepared by immobilizing glycerol dehydrogenase (GDH) on the working electrode area of a bMBZrPRs-SPE, was also assembled. The biosensor was most stable at pH 8.5 (%RSD=5.6, n=70, 0.25 mmol L-1 glycerol). The detection and quantification limits were 2.8x10(-6) and 9.4x10(-6) mol L-1, respectively, and the linear working range was between 1.0x10(-5) and 1.0x10(-4) mol L-1. To assess the effect of interferences, and recovery by the probe we analyzed samples taken during fermentation of chemically defined grape juice medium and compared the results with those obtained by HPLC.},
  author       = {Radoi, A. and Compagnone, D. and Batic, M. and Klincar, J. and Gorton, Lo and Palleschi, G.},
  issn         = {1618-2642},
  keyword      = {NADH,Reinecke salt,zirconium phosphate,meldola blue,glycerol,biosensor},
  language     = {eng},
  number       = {3},
  pages        = {1049--1058},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {NADH screen-printed electrodes modified with zirconium phosphate, Meldola blue, and Reinecke salt. Application to the detection of glycerol by FIA},
  url          = {http://dx.doi.org/10.1007/s00216-006-0975-3},
  volume       = {387},
  year         = {2007},
}