NADH screen-printed electrodes modified with zirconium phosphate, Meldola blue, and Reinecke salt. Application to the detection of glycerol by FIA
(2007) In Analytical and Bioanalytical Chemistry 387(3). p.1049-1058- Abstract
- Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10(-4) mol L-1 NADH, %RSD=4.2, n=90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag-AgCl), flow rate (0.30 mL min(-1)) and pH-dependence... (More)
- Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10(-4) mol L-1 NADH, %RSD=4.2, n=90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag-AgCl), flow rate (0.30 mL min(-1)) and pH-dependence (range 4.0-10.0) were evaluated and optimized. A glycerol biosensor, prepared by immobilizing glycerol dehydrogenase (GDH) on the working electrode area of a bMBZrPRs-SPE, was also assembled. The biosensor was most stable at pH 8.5 (%RSD=5.6, n=70, 0.25 mmol L-1 glycerol). The detection and quantification limits were 2.8x10(-6) and 9.4x10(-6) mol L-1, respectively, and the linear working range was between 1.0x10(-5) and 1.0x10(-4) mol L-1. To assess the effect of interferences, and recovery by the probe we analyzed samples taken during fermentation of chemically defined grape juice medium and compared the results with those obtained by HPLC. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/676456
- author
- Radoi, A. ; Compagnone, D. ; Batic, M. ; Klincar, J. ; Gorton, Lo LU and Palleschi, G.
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- NADH, Reinecke salt, zirconium phosphate, meldola blue, glycerol, biosensor
- in
- Analytical and Bioanalytical Chemistry
- volume
- 387
- issue
- 3
- pages
- 1049 - 1058
- publisher
- Springer
- external identifiers
-
- wos:000243815500033
- scopus:33846592203
- pmid:17203252
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-006-0975-3
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 98148236-b077-4880-82c4-1c1ee8a51b69 (old id 676456)
- date added to LUP
- 2016-04-01 12:10:00
- date last changed
- 2022-02-11 02:59:18
@article{98148236-b077-4880-82c4-1c1ee8a51b69, abstract = {{Bulk screen-printed electrodes (bSPEs) modified with zirconium phosphate (ZrP) and Meldola blue (MB) and by electrochemical deposition of a Reineckate film (bMBZrPRs-SPEs) have been constructed and used as NADH sensors. Cyclic voltammetric investigation of these bulk electrochemically modified screen-printed electrodes revealed stable catalytic activity in oxidation of the reduced form of the coenzyme nicotinamide adenine dinucleotide (NADH). Flow-injection analysis (FIA) coupled with amperometric detection confirmed the improved stability of the bMBZrPRs-SPEs (10(-4) mol L-1 NADH, %RSD=4.2, n=90, pH 7.0). Other conditions, for example applied working potential (+50 mV relative to Ag-AgCl), flow rate (0.30 mL min(-1)) and pH-dependence (range 4.0-10.0) were evaluated and optimized. A glycerol biosensor, prepared by immobilizing glycerol dehydrogenase (GDH) on the working electrode area of a bMBZrPRs-SPE, was also assembled. The biosensor was most stable at pH 8.5 (%RSD=5.6, n=70, 0.25 mmol L-1 glycerol). The detection and quantification limits were 2.8x10(-6) and 9.4x10(-6) mol L-1, respectively, and the linear working range was between 1.0x10(-5) and 1.0x10(-4) mol L-1. To assess the effect of interferences, and recovery by the probe we analyzed samples taken during fermentation of chemically defined grape juice medium and compared the results with those obtained by HPLC.}}, author = {{Radoi, A. and Compagnone, D. and Batic, M. and Klincar, J. and Gorton, Lo and Palleschi, G.}}, issn = {{1618-2642}}, keywords = {{NADH; Reinecke salt; zirconium phosphate; meldola blue; glycerol; biosensor}}, language = {{eng}}, number = {{3}}, pages = {{1049--1058}}, publisher = {{Springer}}, series = {{Analytical and Bioanalytical Chemistry}}, title = {{NADH screen-printed electrodes modified with zirconium phosphate, Meldola blue, and Reinecke salt. Application to the detection of glycerol by FIA}}, url = {{http://dx.doi.org/10.1007/s00216-006-0975-3}}, doi = {{10.1007/s00216-006-0975-3}}, volume = {{387}}, year = {{2007}}, }